Oryza sativa Japonica Group
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA386172 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR5526310.fastq.gz | Fastqsanger.gz | |||
| SRR5526311.fastq.gz | Fastqsanger.gz | |||
| SRR5526312.fastq.gz | Fastqsanger.gz | |||
| SRR5526313.fastq.gz | Fastqsanger.gz | |||
| SRR5526304.fastq.gz | Fastqsanger.gz |
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Project description:WRKY transcriptional factors play important roles in response to various abiotic stresses. In this study, we demonstrate that inserting a copy of soybean WRKY54 driven by a constitutive promoter of CMP (cestrum yellow leaf curling virus) or a drought induced promoter of RD29a, improves drought tolerance in transgenic soybean plants. GmWRKY54 is a transcriptional activator and regulates expressions of large numbers of stress-related genes. Using a GO enrichment toolkit, we found that GmWRKY54 up-regulated GO terms related to transcriptional regulator and gene expression and down-regulated GO terms related to photosynthesis and membrane. Using a co-expression network analysis, we find that dozens of genes are negatively co-expressing with photosynthetic genes. These genes are related to ABA signaling pathway, Ca2+ signaling pathway and transcriptional factors. Transgenic plants confer drought tolerance through improving stomatal closure, reducing transpiration rate and reducing membrane damage during water deficit. GmWRKY54 activates expression of these ABA or Ca2+ signaling-related genes, which finally regulates stomatal aperture. GmWRKY54 binds to the promoter regions of genes encoding an ABA receptor (PYL8-1), two calcium dependent protein kinase (CPK3-1 and CPK3-2), a CBL (Calcineurin B-like protein) interacting protein kinase (CIPK11-2), a SnRK family protein kinase and a ferritin, suggesting that they are the direct targets of GmWRKY54. Our study reveals that GmWRKY54 significantly improves drought tolerance in soybean and regulates expression of large numbers of drought-related genes, suggesting that GmWRKy54 maybe a master regulator of drought response.
2018-05-31 | GSE98958 | GEO
Project description:AREB1deltaQT is a constitutively active form of AREB1, an abscisic acid (ABA)-responsive element binding protein (Uno et al., 2000, PNAS 97: 11632-11637). AREB1 is a bZIP transcription factor in Arabidopsis plants (Col). transcription of the AREB1 gene is upregulated by drought, NaCl, and ABA treatment in vegetative tissues. Because expression of the intact AREB1 gene on its own is insufficient to lead to expression of downstream genes under normal growth conditions, we created an activated form of AREB1 (AREB1deltaQT) to overcome the masked transactivation activity of AREB1. In this experiment, to help to identify target genes of AREB1, we compared the expression profiles in two independent lines 35S-AREB1deltaQT plants under non-stress conditions with that of wild-type plants by using Agilent near-full-genome gene chips (Arabidopsis 22K).
2006-08-02 | E-MEXP-397 | biostudies-arrayexpress
Project description:Drought is one of the major abiotic stresses threatening rice (Oryza sativa) production worldwide. Drought resistance is controlled by multiple genes, and therefore, a multi-gene genetic engineering strategy is theoretically useful for improving drought resistance. However, the experimental evidence for such a strategy is still lacking. In this study, a few drought-responsive genes from rice were assembled by a multiple-round site-specific assembly (MISSA) system, and the constructs were introduced into the rice cultivar KY131 via Agrobacterium-mediated transformation. The transgenic lines of the multi-gene and corresponding single-gene constructs were pre-evaluated for drought resistance. We found that the co-overexpression of two genes, encoding a constitutively active form of a bZIP transcription factor (OsbZIP46CA1) and a protein kinase (SAPK6) involved in the abscisic acid (ABA) signaling pathway, showed significantly enhanced drought resistance compared with the single-gene transgenic lines and the negative transgenic plants. Single-copy lines of this bi-gene combination (named XL22) and the corresponding single-gene lines were further evaluated for drought resistance in the field using agronomical traits. The results showed that XL22 exhibited greater yield, biomass, spikelet number, and grain number under moderate drought stress conditions. The seedling survival rate of XL22 and the single-gene overexpressors after drought stress treatment also supported the drought resistance results. Furthermore, expression profiling by RNA-Seq revealed that many genes involved in the stress response were specifically up-regulated in the drought-treated XL22 lines and some of the stress-related genes activated in CA1-OE and SAPK6-OE were distinct, which could partially explain the different performances of these lines with respect to drought resistance. In addition, the XL22 seedlings showed improved tolerance to heat and cold stresses. Our results demonstrate that the multi-gene assembly in an appropriate combination may be a promising approach in the genetic improvement of drought resistance.
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Project description:Responses to overexpression of wild type and constitutively active Arabidopsis MAP Kinase Kinase 2
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Project description:Plants have evolved to possess adaptation mechanism to cope with drought stress by reprograming transcriptional networks through drought responsive transcription factors, which in turn mediate morphological and physiological changes. NAM, ATAF1-2, and CUC2 (NAC) transcription factors are known to be associated with various developmental processes and stress tolerance. In this study, we functionally characterized the rice drought responsive NAC transcription factor OsNAC14. OsNAC14 was predominantly induced at meiosis stage, and induced by drought, high salinity, ABA and low temperature in leaves than roots. Overexpression of OsNAC14 resulted in drought tolerance at the vegetative growth stage and enhanced filling rate at vegetative growth. OsNAC14 overexpression elevated expression of genes related to DNA damage repair, defense response, strigolactone biosynthesis, which correlated with resistance to drought tolerance. Furthermore, OsNAC14 directly bound to the promoter of drought inducible OsRAD51A1, a key component in homologous recombination in DNA repair system. These results indicate that OsNAC14 mediate drought tolerance by recruiting factors involved in DNA damage repair and defense response to enable plant to protect from cellular damage caused by drought stress, thereby provide mechanism for drought tolerance.
2018-03-21 | GSE106150 | GEO
Project description:Jasmonates (JA) and abscisic acid (ABA) are phytohormones known to play important roles in plant response and adaptation to various abiotic stresses including salinity, drought, wounding, and cold. JAZ (JASMONATE ZIM-domain) proteins have been reported to play negative roles in JA signaling. However, direct evidence is still lacking that JAZ proteins regulate drought resistance. In this study, OsJAZ1 was investigated for its role in drought resistance in rice. Expression of OsJAZ1 was strongly responsive to JA treatment, and it was slightly responsive to ABA, salicylic acid, and abiotic stresses including drought, salinity, and cold. The OsJAZ1-overexpression rice plants were more sensitive to drought stress treatment than the wild-type rice Zhonghua 11 (ZH11) at both the seedling and reproductive stages, while the jaz1 T-DNA insertion mutant plants showed increased drought tolerance compared to the wild-type plants. The OsJAZ1-overexpression plants were hyposensitive to MeJA and ABA, whereas the jaz1 mutant plants were hypersensitive to MeJA and ABA. In addition, there were significant differences in shoot and root length between the OsJAZ1 transgenic and wild-type plants under the MeJA and ABA treatments. A subcellular localization assay indicated that OsJAZ1 was localized in both the nucleus and cytoplasm. Transcriptome profiling analysis by RNA-seq revealed that the expression levels of many genes in the ABA and JA signaling pathways exhibited significant differences between the OsJAZ1-overexpression plants and wild-type ZH11 under drought stress treatment. Quantitative real-time PCR confirmed the expression profiles of some of the differentially expressed genes, including OsNCED4, OsLEA3, RAB21, OsbHLH006, OsbHLH148, OsDREB1A, OsDREB1B, SNAC1, and OsCCD1. These results together suggest that OsJAZ1 plays a role in regulating the drought resistance of rice partially via the ABA and JA pathways.
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Project description:High temperature and drought are the primary yield-limiting environmental constraints for staple food crops. Heat shock transcription factors (HSF) terminally regulate the plant abiotic stress responses to maintain growth and development under extreme environmental conditions. HSF genes of Subclass A2 predominantly express under heat stress (HS) and activate the transcriptional cascade of defense-related genes. In this study, a highly heat-inducible HSF, HvHSFA2e was constitutively expressed in barley (Hordeum vulgareL.) to investigate its role in abiotic stress response and plant development. Transgenic barley plants displayed enhanced heat and drought tolerance in terms of increased chlorophyll content, improved membrane stability, reduced lipid peroxidation, and less accumulation of ROS in comparison to wild-type (WT) plants. Transcriptome analysis revealed that HvHSFA2e positively regulates the expression of abiotic stress-related genes encoding HSFs, HSPs, and enzymatic antioxidants, contributing to improved stress tolerance in transgenic plants. The major genes of ABA biosynthesis pathway, flavonoid, and terpene metabolism were also upregulated in transgenics. Our findings show that HvHSFA2e mediated upregulation of heat-responsive genes, modulation in ABA and flavonoid biosynthesis pathways enhance drought and heat stress tolerance.
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