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Vitis vinifera

ABSTRACT: RNASeq analysis of Vitis vinifera (cv. Sauvignon blanc) grape berries exposed to elevated light

PROVIDER: PRJNA386484 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5534890_1.fastq.gz	Fastqsanger.gz
	SRR5534890_2.fastq.gz	Fastqsanger.gz
	SRR5534900_1.fastq.gz	Fastqsanger.gz
	SRR5534900_2.fastq.gz	Fastqsanger.gz
	SRR5534910_1.fastq.gz	Fastqsanger.gz

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Vitis vinifera Transcriptome or Gene expression

Project description:Seedless varieties are of particular importance to the table-grape and raisin industries. Gibberellin (GA) application is widely used in the early stages of seedless berry development to increase berry size and economic value. However, the underlying mechanism of GA induction of berry enlargement is not well understood. Here, RNA-sequencing analysis of âCentennial Seedlessâ (Vitis vinifera L.) berries treated with GA3 12 days after flowering is reported.

2014-12-05 | E-MTAB-4247 | biostudies-arrayexpress

Vitis vinifera cultivar:Sauvignon Blanc

Project description:Vitis vinifera cv. Sauvignon Blanc Genome sequencing

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Light-induced variation in phenolic compounds in Cabernet Sauvignon grapes (Vitis vinifera L.) involves extensive transcriptome reprogramming of biosynthetic enzymes, transcription factors, and phytohormonal regulators

Project description:Light environments have long been known to influence grape (Vitis vinifera L.) berry development and biosynthesis of phenolic compounds, and ultimately affect wine quality. Here, the accumulation and compositional changes of hydroxycinnamic acids (HCAs) and flavonoids, as well as global gene expression were analyzed in Cabernet Sauvignon grape berries under sunlight exposure treatments at different phenological stages. Sunlight exposure did not consistently affect the accumulation of berry skin flavan-3-ol or anthocyanin among different seasons due to climatic variations, but increased HCA content significantly at véraison and harvest, and enhanced flavonol accumulation dramatically with its timing and severity degree trend. As in sunlight exposed berries, a highly significant correlation was observed between the expression of genes coding phenylalanine ammonia-lyase, 4-coumarate: CoA ligase, flavanone 3-hydroxylase and flavonol synthase family members and corresponding metabolite accumulation in the phenolic biosynthesis pathway, which may positively or negatively be regulated by MYB, bHLH, WRKY, AP2/EREBP, C2C2, NAC, and C2H2 transcription factors (TFs). Furthermore, some candidate genes required for auxin, ethylene and abscisic acid signal transductions were also identified which are probably involved in berry development and flavonoid biosynthesis in response to enhanced sunlight irradiation. Taken together, this study provides a valuable overview of the light-induced phenolic metabolism and transcriptome changes, especially the dynamic responses of TFs and signaling components of phytohormones, and contributes to the further understanding of sunlight-responsive phenolic biosynthesis regulation in grape berries.

2020-09-02 | GSE157347 | GEO

E-GEOD-98873

Project description:RNASeq analysis of Vitis vinifera (cv. Sauvignon blanc) grape berries exposed to elevated light

2020-04-03 | E-GEOD-98873 | ExpressionAtlas

High-throughput sequencing of small RNAs in Vitis vinifera

Project description:Vitis vinifera endogenous small RNAs Size fractionated small RNA from total RNA extracts of Vitis vinifera leaves, inflorescences, tendrils and small berries were ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

2010-04-29 | E-GEOD-18405 | biostudies-arrayexpress

Grape Bud Dormancy -Vitis vinifera

Project description:Bud endodormancy induction response of two genotypes (Seyval, a hybrid white wine grape and Vitis riparia, PI588259, a native North American grape species) was compared under long (15 h) and short (13 h) photoperiods. Proteins were extracted from both genotypes for all time points and experimental conditions. The proteins were separaed by 2D-PAGE, trypsin digested, and the peptides identified with a MALDI-TOF-TOF mass spectrometer. A master gel was made and mapped with all proteins from both genotypes. The proteins were identified by matching the peptide sequences against the 8X Vitis vinifera grape genome in NCBI. This study was funded by NSF grant DBI064755 and is the result of a collaboration between Dr. Anne Fennell at South Dakota State University and Dr. Grant R. Cramer at the University of Nevada, Reno.

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Vitis vinifera cultivar:Sauvignon Blanc

Project description:Genome sequencing of Vitis vinifera cv. Sauvignon Blanc Clone F4V6

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Response of Vitis vinifera to Eutypa lata infection

Project description:Three grapevines cultivars (Merlot, Cabernet-Sauvignon and Ugni Blanc) were infected by E. lata. The expression profiles of the wood part near the infection point were determined for both infected and non infected plant for each cultivars with Nimblegen microarrays vitis. Three plants were used for biological replicates. Comparisons between infected and non infected conditions allow, for each cultivars, the identifcation of genes which the expression is modified by E. lata.

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Project description:Purpose: The identification of genes in Sauvignon blanc berries which respond to nitrogen fertilization at (v+28) and at (v+35) and the identification of genes potentially involved in 3SH precursors biosynthesis. Methods: Grape berries mRNA profiles of control vine (C) and vine fertilized with 100 U/ha of nitrogen (Soil N100) at (v+28) and at (v+35) in 2013 and 2014 were generated by deep sequencing, in triplicate. Results: Genes in Sauvignon blanc berries at 2 stages ((v+28) and (v+35)) which respond to nitrogen supply were identified and genes potentially involved in 3SH precursors synthesis were described.

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High-throughput SuperSAGE for transcript profiling in berry tissues of Vitis vinifera L. under different light regimes

Project description:SuperSAGE is a method of digital gene expression profiling that allows isolation of 26bp tag fragments from expressed transcripts. Because its tag size is larger than that of conventional SAGE, SuperSAGE allowed a secure tag-to-gene annotation using BLAST search against grape genome databases.Transcript profiles in nine samples of grape berry tissues under different light conditions were obtained by SuperSAGE analysis and used for screening the genes which have co-ordinated transcript profiles with the change in the flavonoid composition in the samples analyzed. Candidate genes related to flavonoid biosynthesis and regulation were identified. Nine different grape samples, i.e., flowers, grape berries of Cabernet Sauvignon at 2, 7, 9 weeks after flowering (WAF), berry skins at 17 days after flowering (DAF) shaded after flowering, and berry skins at 17DAF shaded from flowering to 14DAF and then light exposed, were analyzed.

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