Project description:To further characterize differential expression of miRNA and mRNA levels in livers of 12 weeks old male Tax1BP1 wildtype and knock-out mice, the untreated mice were sacrificed and miRNA and mRNA levels were determined
Project description:To further characterize differential expression of miRNA and mRNA levels in livers of 12 weeks old male Tax1BP1 wildtype and knock-out mice, the untreated mice were sacrificed and miRNA and mRNA levels were determined miRNA was labeled with the Affymetrix FlashTag Biotin HSR RNA Labeling Kit
Project description:A label-free quantitative proteomics experiment was performed to study the impact of new1 knock-out on differential protein expression in S. cerevisiae (baker’s yeast).
Project description:ABHD11 has a acylhydrolase activity. Knock-out of abhd11 increases phospholipids in plant leaves. Wholegenome array was done to check the differential expressed genes in lipid metabolic pathway
Project description:To confirm the lack of expression of most miRNAs in DICER1 knock-out cell lines, we performed miRNA microarray analysis. The purpose of this study is to classify DICER1-dependent Small RNA and independent Small RNA using DICER1 cells using deficient cell lines, and to identify novel small RNA and small RNA processing mechanisms.
Project description:Purpose: To build a differential transcriptome network in Smug1 knock-out HepG2 hepatocarcinoma cells. Methods: Transcriptome analysis by the RNA-seq via mRNA pull-down. Results: We constructed transcriptome from Smug1 knock-out cells by using RNA-seq analysis. Nucleosome and miRNA related genes were highly enriched in Smug1 KO HepG2 cells. Conclusions: Smug1 regulate the gene expression related with nucleosome assembly and histone function.