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ABSTRACT: subtidal biofilm Transcriptome

PROVIDER: PRJNA387511 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5601451_1.fastq.gz	Fastqsanger.gz
	SRR5601451_2.fastq.gz	Fastqsanger.gz
	SRR5601453_1.fastq.gz	Fastqsanger.gz
	SRR5601453_2.fastq.gz	Fastqsanger.gz
	SRR5601455_1.fastq.gz	Fastqsanger.gz

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Project description:Planktonic and biofilm cells of Bacillus cereus ATCC 14579 and ATCC 10987 were studied using microscopy and transcriptome analysis. By microscopy, clear differences could be observed between biofilm and planktonic cells as well as between the two strains. By using hierarchical clustering of the transcriptome data, little difference was observed between the biofilm cells of B. cereus ATCC 14579 and ATCC 10987. Different responses between biofilm and planktonic cells could be identified using transcriptome analysis. Biofilm formation seemed to cause a shift in metabolism with up- or down-regulation of genes involved in different metabolic pathways. Genes involved in motility were down-regulated. No clear up-regulation related to capsular or extracellular polysaccharides was observed. Sporulation was observed in biofilm cells using microscopy, which was corroborated with up-regulation of genes involved in sporulation in biofilm cells. The results obtained in this study provide insight in general and strain specific behavior of B. cereus cells in multicellular communities.

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Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus ATCC 14579

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Project description:Purpose: Study transcriptome differences between biofilm, planktonic and stationary cultures. Methods: Total mRNA from in vitro cultures was extracted and sequenced using Ion Torrent PGM sequencer. Results: Characteristic transcriptomic profile was observed for biofilm, planktonic and stationary cultures. Biofilm and planktonic were similar biological states. Conclusions: Results suggest that H. parasuis F9 has more active metabolism during biofilm or planktonic growth when compared to stationary culture. Some identified membrane-related genes could play an important role in biofilm life.

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Project description:Abstract: Transcriptome analysis was applied to characterize the physiological activities of Pseudomonas aeruginosa grown for three days in drip-flow biofilm reactors. Conventional applications of transcriptional profiling often compare two paired data sets that differ in a single experimentally controlled variable. In contrast this study obtained the transcriptome of a single biofilm state, ranked transcript signals to make the priorities of the population manifest, and compared rankings for a priori identified physiological marker genes between the biofilm and published data sets.

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Physiology of Pseudomonas aeruginosa in Biofilms Revealed by Comparative Transcriptomic Analysis.

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Project description:Through the use of RNA-seq, we compared the transcriptome of Group A Streptococcus (GAS) at multiple biofilm time points to multiple planktonic time points in order to determine genes that are differentially expressed during biofilm growth.

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