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Transcriptome and microRNA data involved in Japanese flounder (Paralichthys olivaceus) albinism

ABSTRACT: Transcriptome and microRNA data involved in Japanese flounder (Paralichthys olivaceus) albinism

PROVIDER: PRJNA388157 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR5615790_1.fastq.gz	Fastqsanger.gz
	SRR5615790_2.fastq.gz	Fastqsanger.gz
	SRR5615930.fastq.gz	Fastqsanger.gz
	SRR5615791_1.fastq.gz	Fastqsanger.gz
	SRR5615791_2.fastq.gz	Fastqsanger.gz

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Project description:In this study, a high-throughput sequencing strategy was employed to identify the mRNA involved in P. olivaceus albinism. Based on P. olivaceus genome, RNA-seq identified 21,787 know genes and 711 new genes by transcripts assembly. Of those, 235 genes exhibited significantly different expression pattern (fold change ≥2 or ≤0.5 and q-value≤0.05), including 194 down-regulated genes and 41 up-regulated genes in albino versus normally pigmented individuals. These genes were enriched to 81 GO terms and 9 KEGG pathways (p≤0.05). Among those, the pigmentation related pathways-Melanogenesis and tyrosine metabolism were contained.

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microRNA data involved in Japanese flounder (Paralichthys olivaceus) albinism

Project description:In this study, a high-throughput sequencing strategy was employed to identify the miRNAs involved in P. olivaceus albinism. High-throughput miRNA sequencing identified a total of 475 miRNAs, including 64 novel miRNAs. Furthermore, 33 differentially expressed miRNAs containing 13 up-regulated and 20 down-regulated miRNAs were identified in albino versus normally pigmented individuals (fold change ≥1.5 or ≤0.67 and p≤0.05).

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Gene expression profiles of vaccinated fish using a long-term efficiency mixed vaccine against Edwardsiella tarda performed by a new designed oligo DNA microarray in Japanese flounder Paralichthys olivaceus

Project description:Gene expression profiles by microarray have contributed for a elucidation of an immune-response and a determination of efficiency in vaccination. Recent day, edwardsielosis have caused a fatal damage in the aquaculture of Japanese flounder, Paralichthys olivaceus. However the formalin killed-cell vaccines made from Edwardsiella tarda isolated same fish species were not efficient. Recent our study revealed the mixed FKC vaccine made from the two different type of E. tarda protected Japanese flounder against Edwardsiella tarda infection for long-term. In this study, we analyzed the immune-response of a vaccinated fish kidney using the mixed FKC vaccine against Edwardsiella tarda with an Agilent custom-oligo DNA microarray on 9,573 probes of Japanese flounder. Our study revealed that the mixed FKC vaccine confered a strong immune-response and keeped a efficient for long-term on Japanese flounder.

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The time-dependent global micro-transcriptome of Japanese flounder in response to Vibrio anguillarum infection

Project description:Japanese flounder (Paralichthys olivaceus) is an economic important aquaculture fish that was susceptible to Vibrio anguillarum. To fully deciphered the molecular mechanisms underlying flounder host defense against V. anguillarum infection, we perform the micro-transcriptome analysis of founder spleen with and without V. anguillarum infection at 3 time points.

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Project description:Transcriptome data involved in Japanese flounder (Paralichthys olivaceus) albinism

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