Project description:in vivo microarray study of transcriptional changes of jejunal scratchings (mucosa) obtained from pigs divergent in feed efficiency.
Project description:in vivo microarray study of transcriptional changes of duodenum scratchings (mucosa) obtained from pigs divergent in feed efficiency.
Project description:in vivo microarray study to detect transcriptional changes in muscle tissue (M. longissimus) derived from pigs divergent in feed efficiency.
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig