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Comparative analysis of mouse and human placentae across gestation reveals species-specific regulators of placental development

ABSTRACT: Comparative analysis of mouse and human placentae across gestation reveals species-specific regulators of placental development

PROVIDER: PRJNA390555 | ENA |

REPOSITORIES: ENA

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Comparative analysis of mouse and human placentae across gestation reveals species-specific regulators of placental development [mouse]

Project description:In this study, we compared the genome-wide transcriptome of mouse and human placentas across gestation to identify species-specific signatures of early development. We also compared human placental signatures to purified primary cytotrophoblasts (CTB) isolated from placentae at different gestational age.

2018-02-15 | GSE100052 | GEO

Comparative analysis of mouse and human placentae across gestation reveals species-specific regulators of placental development [Primary CTB]

2018-02-15 | GSE107824 | GEO

Comparative analysis of mouse and human placentas across gestation reveals species-specific regulators of placental development [human]

2018-02-15 | GSE100051 | GEO

Comparative analysis of mouse and human placentae across gestation reveals species-specific regulators of placental development [Primary CTB]

Project description:Comparative analysis of mouse and human placentae across gestation reveals species-specific regulators of placental development [Primary CTB]

| PRJNA421496 | ENA

Comparative analysis of mouse and human placentae across gestation reveals species-specific regulators of placental development.

Project description:An increasing body of evidence points to significant spatio-temporal differences in early placental development between mouse and human, but a detailed comparison of placentae in these two species is missing. We set out to compare placentae from both species across gestation, with a focus on trophoblast progenitor markers. We found that CDX2 and ELF5, but not EOMES, are expressed in early post-implantation trophoblast subpopulations in both species. Genome-wide expression profiling of mouse and human placentae revealed clusters of genes with distinct co-expression patterns across gestation. Overall, there was a closer fit between patterns observed in the placentae when the inter-species comparison was restricted to human placentae through gestational week 16 (thus, excluding full-term samples), suggesting that the developmental timeline in mouse runs parallel to the first half of human placental development. In addition, we identified VGLL1 as a human-specific marker of proliferative cytotrophoblast, where it is co-expressed with the transcription factor TEAD4. As TEAD4 is involved in trophectoderm specification in the mouse, we posit a regulatory role for VGLL1 in early events during human placental development.

| S-EPMC5825847 | biostudies-literature

Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae From Mid-Gestating Pigs

Project description:RNA sequencing was performed to quantify differential gene expression between pig placentae exposed to thermoneutral control or cyclic heat stress conditions between d40 and d60 of gestation (early-mid gestation). Placental samples from female fetuses were collected at d60 of gestation and were subjected to sequencing library preparation and NovaSeq6000 sequencing. 150 bp paired-end reads were generated from each sample. A total of 169 genes were differentially expressed between control and heat stress placentae, of which 35 genes were upregulated and 134 genes were downregulated by maternal heat stress. Gene ontology and pathway enrichment analysis revealed the differentially expressed genes are associated with placental nutrient transporter and metabolism.

2021-04-15 | GSE168571 | GEO

Comparative analysis of mouse and human placentae across gestation reveals species-specific regulators of placental development

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-02-15 | GSE100053 | GEO

Regulation of the human placental (pro)renin receptor-prorenin-angiotensin system by microRNAs

Project description:The placental renin-angiotensin system (RAS) is important for placentation. RAS expression is greatest in early gestation. This may be due (in part) to suppression of miRNAs that target the placental RAS, but this has never been explored. In this study, human placental miRNAs were measured at 10–11 (early), 14–18 (mid), and 38–40 (term) weeks gestation, as well as in placentae from women with early- or late-onset preeclampsia (n=4/group), using an Agilent miRNA microarray (V19). All miRNAs showed a gestational increase and could influence the transgestational profile of the human placental RAS. Additionally, on the array, three miRNAs predicted to target the RAS (miR-892c-3p, miR-378c and miR-514-3p ) were overexpressed in placentae of late-onset preeclamptic women.

2018-08-21 | GSE109832 | GEO

microRNA sequencing of human placentae and matched maternal plasma from 6-23 weeks of gestation

Project description:microRNAs are increasingly seen as important regulators of placental development and opportunistic biomarker targets. We generated miRNA profiles using 96 placentas from presumed normal pregnancies, across early gestation, in combination with matched profiles from maternal plasma. We identified 637 miRNAs with expression in 86 samples (after removing poor quality samples), showing a clear gestational age gradient and identified 374 differentially expressed (DE) miRNAs across a gestational cut-off of 6-10 weeks’ and 11-23 weeks’. We see a clear gestational age group bias in miRNA clusters C19MC, C14MC, miR-17~92 and paralogs, regions that also include many DE miRNAs. Proportional change in expression of placenta-specific miRNA clusters was reflected in maternal plasma, with C19MC miRNAs miR-516b-5p and 517a-3p showing potential as a biomarkers. Chorionic villous samples across early gestation display differential miRNA profiles, particularly in the expression of highly placenta-specific miRNA clusters, and at the presumed introduction of oxygenated maternal blood into the placenta. Data presented here comprise a clinically important reference set for studying early placenta development and enabling development of minimally invasive methods for monitoring placental health.

2021-12-23 | GSE151362 | GEO

Maternal iron deficiency modulates placental transcriptome and proteome in mid-gestation of mouse pregnancy

Project description:Background: Maternal iron deficiency (ID) is associated with poor pregnancy and fetal outcomes. The effect is thought to be mediated by the placenta but there is no comprehensive assessment of placental response to maternal ID. Additionally, whether the influence of maternal ID on the placenta differs by fetal sex is unknown. Objectives: Our primary aim was to identify gene and protein signatures of ID mouse placentas at mid-gestation. A secondary objective was to profile the expression of iron genes in mouse placentas across gestation. Methods: We used a real-time PCR based array to determine the mRNA expression of all known iron genes in mouse placentas at embryonic day (E) 12.5, E14.5, E16.5, and E19.5 (n=3 placentas/time point). To determine the effect of maternal ID, we performed RNA sequencing and proteomics in male and female placentas from ID and iron adequate mice at E12.5 (n=8 dams/diet). Results: In female placentas, six genes including transferrin receptor (Tfrc) and solute carrier family 11 member 2 were significantly changed by maternal ID. An additional 154 genes were altered in male ID placentas. Proteomic analysis quantified 7662 proteins in the placenta. Proteins translated from iron responsive element (IRE) containing mRNAs were altered in abundance; ferritin and ferroportin 1 decreased while TFRC increased in ID placenta. Less than 4% of the significantly altered genes in ID placentas occurred both at the transcriptional and translational levels. Conclusions: Our data demonstrate that the impact of maternal ID on placental gene expression in mice is limited in scope and magnitude at mid-gestation. We provide strong evidence for IRE-based transcriptional and translational coordination of iron gene expression in the mouse placenta. Finally, we discover sexually dimorphic effects of maternal ID on placental gene expression, with more genes and pathways altered in male compared with female mouse placentas.

2021-03-12 | PXD023454 | Pride

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