Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA390771 | ENA |
REPOSITORIES: ENA
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Project description:Endogenous and exogenous extracellular RNAs will be characterized in four body fluids (plasma, serum, CSF and saliva) collected at the same time from 134 healthy subjects ranging in age from 31 to 101 years old. Small RNAs contained in these samples will be profiled by small RNA-seq using unfractionated biofluids and purified extracellular vesicles.
2022-03-30 | GSE199505 | GEO
Project description:Endogenous and exogenous extracellular RNAs will be characterized in four body fluids (plasma, serum, CSF and saliva) collected at the same time from 134 healthy subjects ranging in age from 31 to 101 years old. Small RNAs contained in these samples will be profiled by small RNA-seq using unfractionated biofluids and purified extracellular vesicles.
2022-03-30 | GSE199504 | GEO
Project description:Endogenous and exogenous extracellular RNAs will be characterized in four body fluids (plasma, serum, CSF and saliva) collected at the same time from 134 healthy subjects ranging in age from 31 to 101 years old. Small RNAs contained in these samples will be profiled by small RNA-seq using unfractionated biofluids and purified extracellular vesicles.
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Project description:microRNAs in serum extracellular vesicles in flu-infected mice
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Project description:Transcriptional profiling of Homo sapiens inflammatory skin diseases (whole skin biospies): Psoriasis (Pso), vs Atopic Dermatitis (AD) vs Lichen planus (Li), vs Contact Eczema (KE), vs Healthy control (KO) In recent years, different genes and proteins have been highlighted as potential biomarkers for psoriasis, one of the most common inflammatory skin diseases worldwide. However, most of these markers are not psoriasis-specific but also found in other inflammatory disorders. We performed an unsupervised cluster analysis of gene expression profiles in 150 psoriasis patients and other inflammatory skin diseases (atopic dermatitis, lichen planus, contact eczema, and healthy controls). We identified a cluster of IL-17/TNFα-associated genes specifically expressed in psoriasis, among which IL-36γ was the most outstanding marker. In subsequent immunohistological analyses IL-36γ was confirmed to be expressed in psoriasis lesions only. IL-36γ peripheral blood serum levels were found to be closely associated with disease activity, and they decreased after anti-TNFα-treatment. Furthermore, IL-36γ immunohistochemistry was found to be a helpful marker in the histological differential diagnosis between psoriasis and eczema in diagnostically challenging cases. These features highlight IL-36γ as a valuable biomarker in psoriasis patients, both for diagnostic purposes and measurement of disease activity during the clinical course. Furthermore, IL-36γ might also provide a future drug target, due to its potential amplifier role in TNFα- and IL-17 pathways in psoriatic skin inflammation. In recent years, different genes and proteins have been highlighted as potential biomarkers for psoriasis, one of the most common inflammatory skin diseases worldwide. However, most of these markers are not psoriasis-specific but also found in other inflammatory disorders. We performed an unsupervised cluster analysis of gene expression profiles in 150 psoriasis patients and other inflammatory skin diseases (atopic dermatitis, lichen planus, contact eczema, and healthy controls). We identified a cluster of IL-17/TNFα-associated genes specifically expressed in psoriasis, among which IL-36γ was the most outstanding marker. In subsequent immunohistological analyses IL-36γ was confirmed to be expressed in psoriasis lesions only. IL-36γ peripheral blood serum levels were found to be closely associated with disease activity, and they decreased after anti-TNFα-treatment. Furthermore, IL-36γ immunohistochemistry was found to be a helpful marker in the histological differential diagnosis between psoriasis and eczema in diagnostically challenging cases. These features highlight IL-36γ as a valuable biomarker in psoriasis patients, both for diagnostic purposes and measurement of disease activity during the clinical course. Furthermore, IL-36γ might also provide a future drug target, due to its potential amplifier role in TNFα- and IL-17 pathways in psoriatic skin inflammation.
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Project description:The diagnosis of Parkinson’s disease (PD) lacks precision and occurs relatively late in disease progression. MicroRNAs are epigenetic regulators that have been used as biomarkers in various medical fields. Here, we used small RNA sequencing to quantify the microRNAome of extracellular vesicles isolated from human cerebrospinal fluid (CSF) of PD and control subjects (CTR) aiming to identify a microRNA-based signature for PD.
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Project description:Small extracellular vesicles (sEV) are nano-sized (40-150 nm), membrane-encapsulated vesicles that are released by malignant or pathologic and non-pathologic cells into the extracellular space and function as intercellular signaling vectors through the horizontal transfer of biologic molecules, including microRNA (miRNA) and other small non-coding RNA (ncRNA), that can alter the phenotype of recipient cells. sEV are present in essentially all extracellular biofluids, including serum, urine and saliva, and offer a new avenue for discovery and development of novel biomarkers of various disease states and exposures. The objective of this study was to determine the similarities and differences between sEV ncRNA derived from saliva, serum and urine, as well as cell-free ncRNA from serum. Saliva, urine and serum were concomitantly collected from 4 healthy donors, and sEV were isolated from each respective biofluid, along with cfRNA from serum. sEVs were isolated from the respective biofluids via differential ultracentrifugation. Small RNA-sequencing was performed on each sample, and cluster analysis was performed based on ncRNA profiles. While some similarities existed in terms of sEV ncRNA cargo across biofluids, there are also notable differences in ncRNA class and ncRNA secretion, with each sEVs in each biofluid bearing a unique ncRNA profile. We conclude that sEV ncRNA cargo varies according to biofluid, so thus should be carefully selected and interpreted when designing translational or epidemiological studies.
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Project description:MicroRNAs expression profile in serum sample of liver disease
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Project description:Extracellular vesicles (EVs) play a crucial role in facilitating intercellular communication. The microRNA profiles carried by EVs often exhibit variations between tumor patients and healthy individuals, making them promising biomarkers. These biomarkers are implicated in oncogenic processes and tumor metastasis upon uptake by recipient cells. To investigate the impact of EV biomarkers on tumorigenesis, we exposed HCT-116 cell lines to EVs isolated from the serum of both tumor patients and healthy individuals. In comparison to cell lines treated with extracellular vesicles (EVs) from healthy individuals and blank controls, cell lines treated with EVs from HCC sources exhibit significant alterations in the expression of certain genes associated with pathways related to liver cancer. Our findings shedlight on the intricate interplay between EVs and tumor progression.
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