Project description:BackgroundWood is a secondary xylem generated by vascular cambium. Vascular cambium activities mainly include cambium proliferation and vascular tissue formation through secondary growth, thereby producing new secondary phloem inward and secondary xylem outward and leading to continuous tree thickening and wood formation. Wood formation is a complex biological process, which is strictly regulated by multiple genes. Therefore, molecular level research on the vascular cambium of different tree ages can lead to the identification of both key and related genes involved in wood formation and further explain the molecular regulation mechanism of wood formation.ResultsIn the present study, RNA-Seq and Pac-Bio Iso-Seq were used for profiling gene expression changes in Eucalyptus urophylla × Eucalyptus grandis (E. urograndis) vascular cambium at four different ages. A total of 59,770 non-redundant transcripts and 1892 differentially expressed genes (DEGs) were identified. The expression trends of the DEGs related to cell division and differentiation, cell wall biosynthesis, phytohormone, and transcription factors were analyzed. The DEGs encoding expansin, kinesin, cycline, PAL, GRP9, KNOX, C2C2-dof, REV, etc., were highly expressed in E. urograndis at three years old, leading to positive effects on growth and development. Moreover, some gene family members, such as NAC, MYB, HD-ZIP III, RPK, and RAP, play different regulatory roles in wood formation because of their sophisticated transcriptional network and function redundantly.ConclusionsThese candidate genes are a potential resource to further study wood formation, especially in fast-growing and adaptable eucalyptus. The results may also serve as a basis for further research to unravel the molecular mechanism underlying wood formation.

| S-EPMC10583469 | biostudies-literature

Soil qualities and change rules of Eucalyptus grandis × Eucalyptus urophylla plantation with different slash disposals.

Project description:Slash disposal changes soil quality by affecting soil properties and nutrient cycling, and the appropriate disposal approaches remain controversial. This work aimed to explore the impact of different slash disposal methods on soil qualities. For this purpose, a Eucalyptus grandis × Eucalyptus urophylla plantation that had been cultivated in 2002 and felled for the third time in 2016 was established in Hezhou City, China. Burning forest (BF, for moderate intensity fire) and no-burning forest (NF) were set in the plantation, and the native evergreen broadleaf forest near the plantation was used as the control (CK). Soils were sampled quarterly in 2017, and 27 indicators that represent soil physical, chemical, and biological properties were analyzed and compared through the analysis of the sustainability index (SI), which adopts five indices to calculate soil quality. The obtained data showed that the indicators of BF and NF, except for the total potassium content, were much lower than those of CK. The physical properties (Max-WHC, CWHC, Min-WHC, MMC, CPD, TPD) of NF were significantly better (29.07%, 30.98%, 29.61%, 52.08%, 21.89%, 19.76%) than those of BF, unlike the chemical properties of BF (SOM, TN, ACa, AFe, AMn, ACu, AZn) were significantly better than those of NF (45.61%, 81.33%, 12.78%, 23.18%, 96.13%, 144.30%, 114.04%). The enzymatic activities of NF (URE, APHO) were significantly better (43.33%, 156.58%)than those of BF, except the activities of INV (- 25.21%). Results of SI showed that the soil quality of CK was much better than that of BF, and NF the worst. But it exhibited the most unevenness of CK, followed by NF, and BF the best. The change rules of BF and NF were contrasting, and soil quality reached the same level after half a year. In summary, the soil qualities, either BF or CK, were not comparable to that of CK. BF increased the soil quality fleetly and transiently, and NF was sustainable for the eucalyptus plantation.

| S-EPMC9722714 | biostudies-literature

Effects of polyploidization on gene expression in Eucalyptus urophylla leaves

Project description:In order to study the effect of polyploidization on gene expression in the leaves of Eucalyptus urophylla, triploid obtained by sexual polyploidization and its diploid control were used as materials, and leaves at different growth stages of different ploidies were collected for transcriptome sequencing.

2022-07-13 | GSE207860 | GEO

Eucalyptus urophylla x Eucalyptus tereticornis

Project description:Eucalyptus urophylla x Eucalyptus tereticornis overview

| PRJNA705790 | ENA