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Opisthorchis viverrini

ABSTRACT: Opisthorchis viverrini Raw sequence reads

PROVIDER: PRJNA392273 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR6374241_1.fastq.gz	Fastqsanger.gz
	SRR6374241_2.fastq.gz	Fastqsanger.gz
	SRR6374242_1.fastq.gz	Fastqsanger.gz
	SRR6374242_2.fastq.gz	Fastqsanger.gz

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Opisthorchis viverrini

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Proteomic analysis of the 15k extracellular vesicles from Opisthorchis viverrini

Project description:The proteomic content of the extracellular vesicles (EVs) released by the liver fluke Opisthorchis viverrini has been addressed in the past, but they have focused on the small population pelleting at 120k vesicles (also named exosomes). Here we provide the first proteomic analysis of vesicles pelleting at 15k (microvesicles) using LC-MS/MS.

2024-09-20 | PXD020356 | Pride

Proteomic analysis of the 120k extracellular vesicles from Opisthorchis viverrini

Project description:The proteomic content of the extracellular vesicles (EVs) released by the liver fluke Opisthorchis viverrini has been addressed in the past. Here we employ a more comprehensive purification method of the 120k subpopulation of EVs and analyze proteins present in different locations of these EVs (including external trypsin-liberated peptides, cargo proteins and membrane proteins) using LC-MS/MS.

2022-03-15 | PXD020345 | Pride

Proteomic characterization of Opisthorchis viverrini excretory/secretory products

Project description:O. viverrini infection is recognised by the WHO as a group 1 biological carcinogen because of its extremely strong link with cholangiocarcinoma (CCA), cancer of the bile ducts. The incidence of CCA associated with liver fluke infection, calculated as an age‐standardized rate, varies by geographical region and other risk factors but exceeds 100 per 100,000 in men and 40 per 100,000 in women in hotspots in northeast Thailand. Any attempts to control liver fluke infection, whether through mass drug administration or ultimately through vaccination requires specific and sensitive diagnostic tools that are readily deployable in the field and easy to use. It is imperative that methods to detect infection are appropriately sensitive and rapid in order to diagnose new cases, assess effectiveness of elimination measures and be applicable to large-scale disease surveillance. Indeed, the WHO recently highlighted the low sensitivity of current diagnostic methods as a barrier to controlling liver fluke infection. Herein, we have leveraged the O. viverrini soluble secreted proteome to help create the first O. viverrini protein microarray.

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The miRNAome of O. viverrini induced cholangiocarcinoma by microarray:

Project description:Cholangiocarcinoma is the second most common primary hepatic malignancy worldwide, with intrahepatic cholangiocarcinoma (ICC) a particularly significant public health problem in Southeast Asia, due to its strong association with the food-borne parasite Opisthorchis viverrini (OV). This manuscript represents the first comprehensive miRNA expression profiling by microarray of the three most common histological grades of OV-induced ICC: moderately differentiated, papillary, and well differentiated tumor tissue. No cohort of miRNAs emerged as commonly dysregulated among these histological grades of OV-induced ICC. Instead, each histological grade of ICC tissue showed a distinct miRNA profile. Moderately differentiated tumor tissue showed both the greatest number and the highest magnitude of miRNA dysregulation, followed by papillary ICC tumor tissue, and differentiated ICC tumor tissue. When ICC tumor tissue was compared to adjacent non-tumor tissue, a remarkable similarity in miRNA dysregulation was observed between these samples, indicative of intrahepatic metastasis. These findings indicate the possibility of determining the histological grade of ICC by profiling miRNA dysregulation, which not only would greatly enhance the molecular diagnosis of ICC, but could even lead to the personalized the treatment for ICC by the early classification of histological grade.

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2024-09-12 | PXD045309 | JPOST Repository

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