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Sordaria macrospora

ABSTRACT: A model filamentous ascomycete fungus used for studying fungal sexual development.

PROVIDER: PRJNA39269 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	CABT02.dat.gz	Other
	CABT02.fasta.gz	Fasta.gz
	CABT02.master.dat	Other
	SRR032970.fastq.gz	Fastqsanger.gz

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Gene expression in developmental mutants of Sordaria macrospora

Project description:The aim of this analysis was to analyze gene expression in developmental mutants of the filamentous ascomycete Sordaria macrospora. RNA was isolated from total mycelia, or young fruiting bodies (protoperithecia) were isolated by laser microdissection and RNA was extracted, amplified and used for RNA-sequencing.

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MNase-seq analysis of wild type and asf1 mutant of Sordaria macrospora

Project description:The aim of this analysis was to analyze nucleosome distribution in the filamentous ascomycete Sordaria macrospora by micrococcal nuclease digestion and sequencing.

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Hi-C analysis of the Sordaria macrospora wild type and developmental mutant delta-asf1 [HiC]

Project description:Chromatin organization of the wild type and delta-asf1 mutant of the filamentous ascomycete Sordaria macrospora was analyzed by Hi-C. The delta-asf1 mutant lacks the conserved histone chaperone ASF1 and is unable to produce sexual fruiting bodies. It was hypothesized that the lack of the histone chaperone ASF1 in the mutant might lead to differences in short- or long-distance interactions of chromatin. Furthermore, it was hypothesized that chromatin organization might change during sexual development in the wild type as a prerequisite for the drastic transcriptome changes during development that were observed in earlier studies.

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Gene expression in total sexual mycelium and perithecia of Sordaria macrospora

Project description:The aim of this analysis was to analyze gene expression in developing fruiting bodies (perithecia) of the filamentous ascomycete Sordaria macrospora, and to identify genes that are differentially regulated in the three developmental mutants delta-spt3, delta-asm2, and delta-asm3.

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Bisulfite sequencing of Sordaria macrospora wild type and asf1 mutant

Project description:The aim of this analysis was to analyze DNA methylation in the wild type and the histone chaperone mutant asf1 of the filamentous fungus Sordaria macrospora.

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Establishment of TurboID proximity proteomics in the filamentous fungus Sordaria macrospora

Project description:Biotin identification (BioID) is a proximity-dependent labeling technique to study protein-protein co-localization in vivo. Although BioID has been applied in animal cells, plants and yeast, the method remained to be established in filamentous fungi. In this study, we established BioID for the filamentous fungus Sordaria macrospora using the well-characterized striatin interacting phosphatase and kinase (STRIPAK) complex as a proof of principle. In detail, the STRIPAK complex interactor 1 (SCI1) was fused to a codon-optimized TurboID biotin ligase. This SCI1-TurboID fusion protein complemented the Δsci1 deletion strain phenotype. The identification of the already known SmSTRIPAK components PRO11, SmMOB3, SmPP2Ac1 and PRO22 in a BioID experiment with SCI1-TurboID demonstrated its successful application in S. macrospora. The technique will provide a powerful proteomics tool for fungal biologists.

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Project description:Combining laser microdissection and RNA-seq to chart the transcriptional landscape of fungal development

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Transcriptome analysis of a Sordaria macrospora nox1 mutant

Project description:The Sordaria macrospora nox1 mutant has a block in sexual development at the stage of protoperithecia formation, and therefore is sterile. The aim of this study was to determine the transcriptome of microdissected protoperithecia from the mutant, as well as the transcriptomes of total RNA from the mutant and the wild type for comparison. The data were then compared with transcriptome data from protoperithecia of the wild type and the developemental mutant pro1; the corresponding data were generated in a previous study (Teichert et al. 2012, BMC Genomics 13: 511, GEO accession GSE33668). The samples (amplified RNA from nox1 microdissected protoperithecia and total RNA from nox1 and wild type mycelia undergoing sexual development) were sequenced as paired-end reads on an Illumina HiSeq 2000, two independent biological replicates for each nox1 sample and one replicate for the wild type.

2014-03-10 | E-GEOD-49363 | biostudies-arrayexpress

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Project description:Filamentous fungus used as a model organism for studying several biological processes

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Transcriptome analysis of a Sordaria macrospora nox1 mutant

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