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Homo sapiens

ABSTRACT: RNA-seq comparison of gene induction in RIG-I stimulated old and young human monocytes

PROVIDER: PRJNA392700 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5795110.fastq.gz	Fastqsanger.gz
	SRR5795111.fastq.gz	Fastqsanger.gz
	SRR5795107.fastq.gz	Fastqsanger.gz
	SRR5795108.fastq.gz	Fastqsanger.gz
	SRR5795109.fastq.gz	Fastqsanger.gz

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RNA-seq comparison of gene induction in RIG-I stimulated old and young human monocytes

Project description:Goal: The goal of this study was to compare gene induction in old and young human monocytes upon stimulation of these cells with a RIG-I specific ligand, based on previously published results which indicated impaired interferon responses in monocytes from older individuals upon influenza virus infeciton.

2017-12-31 | GSE100709 | GEO

Human monocyte subsets are transcriptionally and functionally altered in aging in response to pattern recognition receptor agonists [ExVivo]

Project description:Age-related alterations in immunity have been linked to increased incidence of infections and decreased responses to vaccines in the aging population. Human peripheral blood monocytes are known to promote antigen presentation and antiviral activities; however, the impact of aging on monocyte functions remains an open question. We present an in-depth global analysis examining the impact of aging on classical (CD14+CD16-), intermediate (CD14+CD16+), and non-classical (CD14dimCD16+) monocytes. Monocytes sorted from non-frail healthy adults (18-40 yrs) and old (≥ 65 yrs) individuals were analyzed after stimulation with TLR4, TLR7/8, and RIG-I agonists. Our data showed under non-stimulated conditions, monocyte subsets did not reveal significant age-related alternations; however, agonist stimulated-monocytes from adults and old subjects did show differences at the transcriptional and functional levels. These alternations in many immune-related transcripts and biological processes resulted in reduced production of IFNα, IFN, IL-1β, CCL20, and CCL8, and higher expression of CX3CR1 in monocytes from old subjects. Our findings represent a comprehensive analysis of the influence of human aging on pattern recognition receptors signaling and monocyte functions, and have implications for strategies to enhance the immune response in the context of infection and immunization.

2017-07-27 | GSE94497 | GEO

Human monocyte subsets are transcriptionally and functionally altered in aging in response to pattern recognition receptor agonists [InVitro]

Project description:Age-related alterations in immunity have been linked to increased incidence of infections and decreased responses to vaccines in the aging population. Human peripheral blood monocytes are known to promote antigen presentation and antiviral activities; however, the impact of aging on monocyte functions remains an open question. We present an in-depth global analysis examining the impact of aging on classical (CD14+CD16-), intermediate (CD14+CD16+), and non-classical (CD14dimCD16+) monocytes. Monocytes sorted from non-frail healthy adults (18-40 yrs) and OLD (≥ 65 yrs) individuals were analyzed after stimulation with TLR4, TLR7/8, and RIG-I agonists. Our data showed under non-stimulated conditions, monocyte subsets did not reveal significant age-related alternations; however, agonist stimulated-monocytes from adults and OLD subjects did show differences at the transcriptional and functional levels. These alternations in many immune-related transcripts and biological processes resulted in reduced production of IFNα, IFN, IL-1β, CCL20, and CCL8, and higher expression of CX3CR1 in monocytes from OLD subjects. Our findings represent a comprehensive analysis of the influence of human aging on pattern recognition receptors signaling and monocyte functions, and have implications for strategies to enhance the immune response in the context of infection and immunization.

2017-07-27 | GSE94496 | GEO

Homo sapiens

Project description:Young and old muscle comparison

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Project description:old and young mouse RNA-seq

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The epigenomic landscape of cognitive stimulation in the context of aging in mouse dorsal hippocampus - SWATH

Project description:Aging is a universal biological phenomenon linked to many diseases, such as cancer or neurodegeneration. However, the molecular mechanisms underlying aging, or how lifestyle interventions such as cognitive stimulation can ameliorate this process, are yet to be clarified. Here, we performed a multi-omic profiling, including RNA-seq, ATAC-seq, ChIP-seq, EM-seq, SWATH-MS and single cell Multiome scRNA and scATAC-seq, in the dorsal hippocampus of young and old mouse subjects which were subject to cognitive stimulation using the paradigm of environmental enrichment. In this study we were able to describe the epigenomic landscape of aging and cognitive stimulation.

2023-09-29 | PXD045567 | Pride

Suppression of inflammaging prolongs hematopoietic healthspan [dataset 5]

Project description:The impact of Thbs1 gene deletion on BMEC activity during physiological aging is unknown. In this experiment, RNA Seq analysis was performed on BMECs derived from aged (18 month-old) Thbs1 knockout mice. BMECs derived from young (3 month-old) and aged (18 month-old) control mice were used as controls for comparison.

2025-01-03 | GSE255654 | GEO

Suppression of inflammaging prolongs hematopoietic healthspan [dataset 4]

Project description:The impact of Thbs1 gene deletion on BM MSC activity during physiological aging is unknown. In this experiment, RNA Seq analysis was performed on BM MSCs derived from aged (18 month-old) Thbs1 knockout mice. MSCs derived from young (3 month-old) and aged (18 month-old) control mice were used as controls for comparison.

2025-01-03 | GSE255653 | GEO

Suppression of inflammaging prolongs hematopoietic healthspan [dataset 3]

Project description:The impact of Thbs1 gene deletion on HSC activity during physiological aging is unknown. In this experiment, RNA Seq analysis was performed on BM HSCs derived from aged (18 month-old) Thbs1 knockout mice. HSCs derived from young (3 month-old) and aged (18 month-old) control mice were used as controls for comparison.

2025-01-03 | GSE255652 | GEO

Age of the bone marrow dictates clonality of smooth muscle-derived cells in the atherosclerotic plaque [bulk RNA-seq]

Project description:Purpose: The goal of this study is to investigate the gene expression in aged (18 month old) and young (3 month old) bone marrow-derived monocytes. Methods: Bone marrow was harvested by flushing the femurs and tibias of young (3 month old) and aged (18 month old) mice in PBS with 2% fetal bovine serum (FBS) on ice. Cells were isolated from bone marrow by density gradient centrifugation using Lympholyte (Cedarlane, CL0531). After centrifugation at 1300 g for 20 min, bone marrow cells were collected from the interface and washed in Ca2+/Mg2+ - free HBSS. The cells were dissociated to a single-cell suspension by filtering through a 70-µm nylon mesh. For monocyte isolation, a suspension of bone marrow cells was stained with Alexa 700 anti-CD3 (1:100, BioLegend, 100215), PE-Cy7 anti-CD19 (1:100, BioLegend, 115520), FITC anti-CD11b (1:100, BD Pharmingen, 553310) and APC anti-Ly6c antibodies (1:100, BioLegend, 128015) in PBS with 0.5% FBS for 30 min. CD11b+Ly6c+CD3-CD19- monocytes were isolated with a BD FACSAria cell sorter. Total RNA was extracted and subjected to bulk RNA-seq using Illumina HiSeq 2000. Results: Expression profiles of the transcriptome are compared between bone marrow-derived monocytes isolated from aged (18 month old) and young (3 month old) mice. Conclusions: Significant differences in the expression levels of genes are noted between the two age groups.

2022-08-01 | GSE206429 | GEO

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