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SCD – Stem Cell Differentiation on board the International Space Station: the problem of human bone loss

ABSTRACT: SCD – Stem Cell Differentiation on board the International Space Station: the problem of human bone loss

PROVIDER: PRJNA393449 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5811930.fastq.gz	Fastqsanger.gz
	SRR5811931.fastq.gz	Fastqsanger.gz
	SRR5811932.fastq.gz	Fastqsanger.gz
	SRR5811923.fastq.gz	Fastqsanger.gz
	SRR5811933.fastq.gz	Fastqsanger.gz

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SCD – Stem Cell Differentiation on board the International Space Station [RNA-seq]

Project description:The bone loss observed in astronauts and animal models after spaceflight is attributable to alterations in the bone tissue formation that depends from the continuous remodeling through the activities of bone-resorbing osteoclasts of hematopoietic lineage and bone-forming osteoblasts of mesenchymal origin. This disease is frequent in aged people, but develops much more rapidly in space. Our experiment, selected by ESA (European Space Agency), aimed to determine how human bone marrow mesenchymal stem cells (hBMSCs) react and differentiate in real microgravity, on board the International Space Station, in approx. 2 weeks time.

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Effects of a Closed Space Environment on Gene Expression in Hair Follicles of Astronauts in the International Space Station

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Project description:Efficient generation of functional cardiomyocytes from human induced pluripotent stem cells (hiPSC-CMs) is critical for their use in regenerative medicine and other applications. In this study, we evaluated the effect of space microgravity (µg) on the differentiation of hiPSC-derived cardiac progenitors compared with parallel 1g condition on the International Space Station. Cryopreserved 3D cardiac progenitors derived from hiPSCs were cultured for 3 weeks. Compared with 1g culture, the µg culture had larger sphere sizes, increased expression of proliferation markers, higher counts of nuclei, and higher cell viability. Highly enriched cardiomyocytes generated in µg had appropriate gene expression and cardiac structure as well as improved function including contractility and Ca2+ handling. RNA-seq analysis of 3-day cultures revealed that short-term exposure of cardiac progenitor spheres to space microgravity upregulated genes involved in cell proliferation, cardiac differentiation, and contraction. These results indicate that space microgravity increased survival and proliferation of hiPSC-CMs and improved their structures and functions.

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