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Staphylococcus aureus strain:HG003

ABSTRACT: The nucleoid occlusion factor Noc controls DNA replication initiation in Staphylococcus aureus

PROVIDER: PRJNA393463 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5815600_1.fastq.gz	Fastqsanger.gz
	SRR5815600_2.fastq.gz	Fastqsanger.gz
	SRR5815610_1.fastq.gz	Fastqsanger.gz
	SRR5815610_2.fastq.gz	Fastqsanger.gz
	SRR5815601_1.fastq.gz	Fastqsanger.gz

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The nucleoid occlusion factor Noc controls DNA replication initiation in Staphylococcus aureus

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The nucleoid occlusion factor Noc controls DNA replication initiation in Staphylococcus aureus [ChIP-Seq]

Project description:Successive division events in the spherically shaped bacterium Staphylococcus aureus are oriented in three alternating perpendicular planes. The mechanisms that underlie this relatively unique pattern of division and coordinate it with chromosome segregation remain largely unknown. Thus far, the only known spatial regulator of division in this organism is the nucleoid occlusion protein Noc that inhibits assembly of the cytokinetic ring over the chromosome. However, Noc is not essential in S. aureus, indicating that additional regulators are likely to exist. To search for these factors, we screened for mutants that are synthetic lethal with Noc inactivation. Our characterization of these mutants led to the discovery that S. aureus Noc also controls the initiation of DNA replication. We show that cells lacking Noc over-initiate and mutations in the initiator gene dnaA suppress this defect. Importantly, these dnaA mutations also partially suppress the division problems associated with ∆noc. Reciprocally, we show that over-expression of DnaA enhances the over-initiation and cell division phenotypes of cells lacking Noc. Thus, a single factor both blocks cell division over chromosomes and helps to ensure that new rounds of DNA replication are not initiated prematurely. This degree of economy in coordinating key cell biological processes has not been observed in rod-shaped bacteria and may reflect the challenges posed by the reduced cell volume and complicated division pattern of this spherical pathogen.

2017-08-31 | GSE93264 | GEO

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Chromatin immunoprecipitation of Bacillus subtilis to identify Noc protein binding sites

Project description:Coordination of chromosome segregation and cytokinesis is crucial for efficient cell proliferation. In Bacillus subtilis the nucleoid occlusion protein Noc protects chromosomes by associating with the chromosome and preventing cell division in its vicinity. Using protein localization, ChAP-on-Chip and bioinformatics, we have identified a consensus Noc-binding DNA sequence (NBS), and show that Noc is targeted to about 70 discrete regions scattered around the chromosome, though absent from a large region around the replication terminus. Purified Noc bound specifically to an NBS in vitro. NBSs inserted near the replication terminus bound Noc-YFP and caused a delay in cell division. An autonomous plasmid carrying an NBS recruited Noc-YFP and conferred a severe Noc-dependent inhibition of cell division. This shows that Noc is a potent inhibitor of division but that its activity is strictly localized by interaction with NBS sites in vivo. We propose that Noc not only serves as a spatial regulator of cell division to protect the nucleoid, but also a timing device with an important role in the co-ordination of chromosome segregation and cell division.

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Staphylococcus aureus strain:HG003

Project description:Staphylococcus aureus Tn-Seq

| PRJNA353375 | ENA