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Dynamic gene expression response to altered gravity in human myelomonocytic U937 cells

ABSTRACT: Dynamic gene expression response to altered gravity in human myelomonocytic U937 cells

PROVIDER: PRJNA393985 | ENA |

REPOSITORIES: ENA

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Dynamic gene expression response to altered gravity in human myelomonocytic U937 cells [parabolic]

Project description:We investigated differentially regulated genes in human myelomonocytic U937 cells in 20s and 5min microgravity and in hypergravity and compared expression profiles to identify potential gravity-regulated genes and adaptation processes.

2018-10-16 | GSE101212 | GEO

Dynamic gene expression response to altered gravity in human myelomonocytic U937 cells [sounding rocket]

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Dynamic gene expression response to altered gravity in human myelomonocytic U937 cells

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:We investigated differentially regulated genes in human Jurkat T lymphocytic cells in 20s and 5min microgravity and in hypergravity and compared expression profiles to identify potential gravity-regulated genes and adaptation processes.

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gravity-Gravitational signature of synchronized cell cultures in particular cell cycle stages

Project description:au13-11_gravity - gravity - Cell cycle and cell proliferation are decoupled under altered gravity conditions. We have previously shown that semisolid cell cultures of Arabidopsis suffer overall genome changes in response to altered gravity and also that cell cycle stages duration is altered. By using synchronized cell cultures we will demonstrate the precise alterations in cell cycle duration and also the transcriptional signature in any of them. - Experiments consists on exposures of Arabidopsis cell cultures to 1g control/simulated microgravity (RPM) conditions. Asynchronous cells exposed for 14 h + Syncronous populations choosen to have an enrichment of cell cycle phases were used (being T7/T10 samples on G2 phase, T14/T16 samples on G1 phase). 6 dye-swap - time course,treated vs untreated comparison

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