Project description:Transcriptome comparison of an aldosterone producing adenoma transcriptome and its adjacent zona glomerulosa. Both tissues are from the same individual, are differentiated for aldosterone production, but the adjacent zona glomerulosa produces no aldosterone by negative feedback. Keywords = aldosterone producing adenoma transcriptome Keywords: parallel sample
Project description:Transcriptome comparison of an aldosterone producing adenoma transcriptome and its adjacent zona glomerulosa. Both tissues are from the same individual, are differentiated for aldosterone production, but the adjacent zona glomerulosa produces no aldosterone by negative feedback. Keywords = aldosterone producing adenoma transcriptome Keywords: parallel sample
Project description:To further explore the clinical significance of lncRNAs in aldosterone producing adenoma (APA), we have employed large-scale biological database based transcriptome sequencing and microarray techniques as a discovery platform to identify expression changes of lncRNAs between APA (sample3, 4, 5) and normal adrenal (NA, sample A, B, L) tissue. By microarray analysis, we selected differentially expressed LncRNAs and mRNAs, and we further constructed coding-non-coding gene co-expression networks (CNC network) based on the correlation analysis between lncRNAs and target mRNAs. These networks will subsequently help to predict lncRNAs target genes, and will provide important insights into the role of lncRNAs in APA pathogenesis.
Project description:To investigate the development of aldosterone-producing adenoma, we profiled cells of different areas of the adjacent adrenal cortex by snRNAseq to obtain possible trajectories towards adenoma formation.
Project description:Analysis of aldosterone-producing adenoma (APA) samples from patients with primary hyperaldosteronism. These APAs have a somatic mutation in either KCNJ5, CACNA1D, or ATP1A1. Results provide insight into the different mechanisms each mutation may cause leading to elevated aldosterone production in APA. In this dataset, we include expression data from aldosterone-producing adenomas (APAs) with a somatic mutation in either KCNJ5, CACNA1D, or ATP1A1. These data are used to obtain differentially expressed genes between KCNJ5 mutant APAs and CACNA1D/ATP1A1 mutant APAs.
