Schizosaccharomyces pombe
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA397399 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR5905140.fastq.gz | Fastqsanger.gz | |||
| SRR5905150.fastq.gz | Fastqsanger.gz | |||
| SRR5905160.fastq.gz | Fastqsanger.gz | |||
| SRR5905170.fastq.gz | Fastqsanger.gz | |||
| SRR5905141.fastq.gz | Fastqsanger.gz |
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Project description:Post-translational modifications of the transcription elongation complex provide mechanisms to fine-tune gene expression, yet their specific impacts on RNA polymerase II regulation remain difficult to ascertain. Here, in Schizosaccharomyces pombe, we examine the role of Cdk9, and related Mcs6/Cdk7 and Lsk1/Cdk12 kinases, on transcription at base-pair resolution with Precision Run-On sequencing (PRO-seq). Within a minute of Cdk9 inhibition, phosphorylation of Pol II-associated factor, Spt5 is undetectable. The effects of Cdk9 inhibition are more severe than inhibition of Cdk7 and Cdk12, resulting in a shift of Pol II towards the transcription start site (TSS). A time course of Cdk9 inhibition reveals that early transcribing Pol II can escape promoter-proximal regions, but with a severely reduced rate of only ~400 bp/min. Our results in fission yeast suggest the existence of a conserved global regulatory checkpoint that requires Cdk9 kinase activity.
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