Project description:ra06-04_bos1 - erwinia chrysanthemi treatments - BOS1 signaling pathway in Arabidopsis thaliana / Erwinia chrysanthemi interaction - Comparison of 4 transcriptoms : Wt Arabidopsis ecotype infected by Wt Erwinia chrysanthemi strain, bos1 Arabidopsis mutant infected by Wt Erwinia chrysanthemi strain, Wt Arabidopsis ecotype infected by BOS1-non inducing pelBC Erwinia chrysanthemi mutant, Wt Arabidopsis ecotype buffer inoculated. Experiments were repeated 3 times. Keywords: normal vs disease comparison

2009-06-13 | GSE11793 | GEO

erwinia chrysanthemi treatments-BOS1 signaling pathway in Arabidopsis thaliana / Erwinia chrysanthemi interaction

2009-06-13 | E-GEOD-11793 | biostudies-arrayexpress

Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization.

Project description:BackgroundAndrimid is reported to be a novel kind of polyketide-nonribosomal peptide hybrid product (PK-NRPs) that inhibits fatty acid biosynthesis in bacteria. Considering its great potential in biomedicine and biofarming, intensive studies have been conducted to increase the production of andrimid to overcome the excessive costs of chemosynthesis. In screening for species with broad-spectrum antibacterial activity, we detected andrimid in the fermentation products of Erwinia persicina BST187. To increase andrimid production, the BST187 fermentation medium formulation and fermentation conditions were optimized by using systematic design of experiments (One-Factor-At-A-Time, Plackett-Burman design, Response Surface Methodology).ResultsThe results indicate that the actual andrimid production reached 140.3 ± 1.28 mg/L under the optimized conditions (trisodium citrate dihydrate-30 g/L, beef extract-17.1 g/L, MgCl2·6H2O-100 mM, inoculation amount-1%, initial pH-7.0, fermentation time-36 h, temperature-19.7℃), which is 20-fold greater than the initial condition without optimization (7.00 ± 0.40 mg/L), consistent with the improved antibacterial effect of the fermentation supernatant.ConclusionsThe present study provides valuable information for improving andrimid production via optimization of the fermentation process, which will be of great value in the future industrialization of andrimid production.

| S-EPMC10519088 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data