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Arabidopsis thaliana

ABSTRACT: Comparison of sRNA populations obtained using HD adapters in A thaliana, with NEB RecJ exonuclease or Superscript II enzymes

PROVIDER: PRJNA397910 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR5926200.fastq.gz	Fastqsanger.gz
	SRR5926201.fastq.gz	Fastqsanger.gz
	SRR5926202.fastq.gz	Fastqsanger.gz
	SRR5926203.fastq.gz	Fastqsanger.gz

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Comparison of sRNA populations obtained using HD adapters in A thaliana, with NEB RecJ exonuclease or Superscript II enzymes

Project description:Multiple companies produce RecJ exonuclease and RNA dependent DNA polymerase for making cDNA. Our standard protocol was developed based on the enzymes from Epicenter (epicenter was recently bought by the Illumina company). As an alternative for the enzymes from EPicenter, we tested the RecJ exonuclease from NEB and Superperscript II from Invitrogen in sRNA library construction using HD adapters [Sorefan et al 2012, Xu et al 2015].

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Project description:The sRNA population (sequence identity and distribution of abundances) in A thaliana was assessed using High Definition (HD) [Sorefan et al 2012, Xu et al 2015] or standard Illumina adapters; the usage of HD adapters revealed a more diverse population of sRNAs (in particular, it revealed the presence of more unique 24mers)

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Project description:The sRNA population (sequence identity and distribution of abundances) in S Lycopersicum was assessed using High Definition (HD) [Sorefan et al 2012, Xu et al 2015] or standard Illumina adapters; the usage of HD adapters revealed a more diverse population of sRNAs (in particular, it revealed the presence of more unique 24mers)

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