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Homo sapiens

ABSTRACT: Transcriptome profiling of human erythroblast cells knocking down NCOA4

PROVIDER: PRJNA398102 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5929673_1.fastq.gz	Fastqsanger.gz
	SRR5929673_2.fastq.gz	Fastqsanger.gz
	SRR5929674_1.fastq.gz	Fastqsanger.gz
	SRR5929674_2.fastq.gz	Fastqsanger.gz
	SRR5929675_1.fastq.gz	Fastqsanger.gz

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Transcriptome profiling of human erythroblast cells knocking down NCOA4

Project description:Purpose: Knocking down NCOA4 disrupted Thyroid hormone receptor beta agonist,GC-1, mediated terminal human erythroblast differentiation. Therefore, we conducted RNA-seq to profile the transcriptome changes in NCOA4 knocked down human erythroblasts.. Methods: Human CD34+ cells were transduced by lentiviruses encoding shRNAs targeting either LacZ (control) or NCOA4 atday 1 of culture. At day 14, cells were switched to terminal differentiation medium. Results: Using an optimized data analysis workflow, we have identified an ensemble of genes whose expression are discrupted by NCOA4 knockdown. Conclusions: There is a significant correlation between the degree of GC-1-mediated gene activation and the degree of NCOA4 knockdown-mediated gene repression.

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