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Clematis terniflora

ABSTRACT: Clematis terniflora DC. RNA-Seq treated with UV-B radiation followed by dark

PROVIDER: PRJNA399601 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5957665_1.fastq.gz	Fastqsanger.gz
	SRR5957665_2.fastq.gz	Fastqsanger.gz
	SRR5957666_1.fastq.gz	Fastqsanger.gz
	SRR5957666_2.fastq.gz	Fastqsanger.gz
	SRR5957667_1.fastq.gz	Fastqsanger.gz

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Integrative proteomic and phosphoproteomic analyses revealed the regulation mechanism of Clematis terniflora DC. response to UV-B stress

Project description:Clematis terniflora DC. is used as traditional herb medicine since ancient China. The active substances in C.terniflora have been confirmed to be effective in treating disease like prostatitis. The UV light radiation is a common environmental factor that damage the plants and has an influence on the primary metabolism and secondary metabolism. Previous researches have shown that ultraviolet B (UV-B) radiation followed by dark stress acting on C.terniflora leaves，resulting in the accumulation of secondary metabolites. An in-depth understanding of how C.terniflora leaves respond to UV-B stress is crucial for improving medicinal plants value. Here, we conducted label-free proteomic and phosphoproteomic to explore the protein changes under UV-B and UV-B combined with dark treatment, respectively. A total of 2839 proteins and 1638 phosphorylated proteins were identified. Integrative omics revealed the photosynthetic system and carbohydrate balance were modulated in both stresses. The phosphoproteomic data indicated that, mitogen-activated protein kinase signaling pathway was triggered, meanwhile, the abundance of phosphorylated proteins related to the osmotic stress were increased under UV-B stress. Differential abundant phosphoproteins from UV-B followed by dark treatment were mainly enriched in response to stimulus including calcium-mediated proteins. This study dissects the impact of UV-B stress on C.terniflora and the plant molecular resistant mechanisms through proteomic and phosphoproteomic in a new sight.

2023-03-11 | PXD038136 | Pride

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Comparative Proteomic Analysis Reveals Elevated Capacity for Photosynthesis in Polyphenol Oxidase Expression-Silenced Clematis terniflora DC. Leaves

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