Project description:Relative quantification of protein abundances of three yeast strains (Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS6556 and Yarrowia lipolytica W29) cultivate in chemostats under different conditions. The conditions for Saccharomyces cerevisiae CEN.PK113-7D are: - Standard condition – 30°C, pH 5.5 - High temperature - 36°C, pH 5.5 - Low pH - 30°C, pH 3.5 - Osmotic stress – 30°C, pH 5.5, 1M KCl The conditions for Kluyveromyces marxianus CBS6556 are: - Standard condition – 30°C, pH 5.5 - High temperature - 40°C, pH 5.5 - Low pH - 30°C, pH 3.5 - Osmotic stress – 30°C, pH 5.5, 0.6 M KCl The conditions for Yarrowia lipolytica W29 are: - Standard condition - 28°C, pH 5.5 - High temperature - 32°C, pH 5.5 - Low pH - 28°C, pH 3.5 This study is part of the OMICS data generation of CHASSY project (European Union’s Horizon 2020 grant agreement No 720824).

2019-11-11 | PXD011426 | Pride

Approaching absolute quantification from chemostats cultures of Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS 6556 and Yarrowia lipolytica W29

Project description:Protein extracts of three yeast strains (Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS6556 and Yarrowia lipolytica W29) cultivated in chemostats under different conditions. Representative samples containing aliquots of all conditions for each yeast strain were spiked with UPS2 standard (Sigma) to estimate absolute values in fmol. The conditions for Saccharomyces cerevisiae CEN.PK113-7D are: - Standard condition : 30°C, pH 5.5 - High temperature: 36°C, pH 5.5 - Low pH: 30°C, pH 3.5 - Osmotic stress : 30°C, pH 5.5, 1M KCl The conditions for Kluyveromyces marxianus CBS6556 are: - Standard condition : 30°C, pH 5.5 - High temperature: 40°C, pH 5.5 - Low pH: 30°C, pH 3.5 - Osmotic stress: 30°C, pH 5.5, 0.6 M KCl The conditions for Yarrowia lipolytica W29 are: - Standard condition: 28°C, pH 5.5 - High temperature: 32°C, pH 5.5 - Low pH: 28°C, pH 3.5 This study is part of the OMICS data generation WP of CHASSY project (European Union’s Horizon 2020 grant agreement No 720824).

2022-02-15 | PXD012836 | Pride

RNA sequencing experiment of S. Cerevisiae strain CEN.PK113-7D in four different chemostat conditions

Project description:The RNA sequencing experiment was performed in four different chemostat conditions as control for a CAGE sequencing experiment performed in the same conditions using the industrial relevant S. Cerevisiae strain CEN.PK113-7D

2019-01-03 | E-MTAB-6722 | biostudies-arrayexpress

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