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Porphyromonas gingivalis W83

ABSTRACT: Expression profiling analyses of ECF sigma factor PG0162-PG1660 mutant of Porphyromonas gingivalis W83 [RNA-Seq]

PROVIDER: PRJNA406902 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6028661.fastq.gz	Fastqsanger.gz
	SRR6028662.fastq.gz	Fastqsanger.gz
	SRR6028663.fastq.gz	Fastqsanger.gz
	SRR6028664.fastq.gz	Fastqsanger.gz
	SRR6028665.fastq.gz	Fastqsanger.gz

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Expression profiling analyses of ECF sigma factor PG1660 mutant of Porphyromonas gingivalis W83

Project description:To study the expression profile of ECF sigma factor PG1660 mutant under anaerobic conditions and hydrogen peroxide stress conditions compared to the wild-type W83 by using DNA-microarray. The role of ECF sigma factor PG1660 involved in oxidative stress was published Yuetan Dou, Devon Osbourne, Rachelle McKenzie, Hansel M Fletcher. (2010) Involvement of extracytoplasmic function sigma factors in virulence regulation in Porphyromonas gingivalis W83. FEMS Microbiology Letter, 312(1):24-32.

2017-09-14 | GSE103838 | GEO

Expression profiling analyses of ECF sigma factor PG0162-PG1660 mutant of Porphyromonas gingivalis W83 [RNA-Seq]

Project description:The role of ECF sigma factors PG0162, PG01660 were involved in virulence regulationin Porphyromonas gingivalis was published Yuetan Dou, Devon Osbourne, Rachelle McKenzie, Hansel M Fletcher. (2010) Involvement of extracytoplasmic function sigma factors in virulence regulation in Porphyromonas gingivalis W83. FEMS Microbiology Letter, 312(1):24-32.

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Comparison of Porphyromonas gingivalis W83 gene expression when grown with or without galactose

Project description:Porphyromonas gingivalis (P. gingivalis) W83 cultures in early exponential phase were supplemented with 0.1% galactose or vehicle control. The cultures were then permitted to grow anaerobically at 37°C for 3 hours, which corresponds to approximately one doubling of P. gingivalis. At this point, RNA extraction was performed using the cultures, and these samples were processed and submitted for RNA sequencing using an Illumina platform. Modest changes in gene expression were observed between cultures grown with or without galactose, and the majority of changes were associated with processes occurring at the cell membrane.

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Gene expression changes in Porphyromonas gingivalis W83 after inoculation in rat oral cavity

Project description:The aim of this study is to investigate the alterations in gene expression in Porphyromonas gingivalis W83 after inoculation in rat oral cavity. P.gingivalis W83 inoculation in rat oral cavity caused inflammatory responses in gingival tissues and destroyed host alveolar bone. Microarray analysis revealed that 42 genes were upregulated, and 22 genes were downregulated in the detected 1786 genes in the inoculated P.gingivalis W83. Products of these upregulated and downregulated genes are mainly related to transposon functions, cell transmembrane transportation, protein and nucleic acid metabolism, energy metabolism, cell division and bacterial pathogenicity.P.gingivalis W83 has a pathogenic effect on host oral cavity. Meanwhile, inflammatory oral environment alters P.gingivalis W83 gene expression profile. These changes in gene expression may limit the proliferation and weaken the pathogenicity of P.gingivalis W83, and favor themselves to adapt local environment for survival.

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