Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Arabidopsis thaliana

ABSTRACT: Genomewide identification of LHY binding targets

PROVIDER: PRJNA407041 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6031857.fastq.gz	Fastqsanger.gz
	SRR6031858.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Arabidopsis thaliana

Project description:Genomewide identification of LHY binding targets.

| PRJNA227097 | ENA

Genomewide identification of ANAC060 binding targets

Project description:Genomewide identification of ANAC060 binding targets

| PRJNA507758 | ENA

Arabidopsis thaliana

Project description:Identification of WUSCHEL direct targets.

| PRJNA140197 | ENA

Arabidopsis thaliana

Project description:Identification of AtGRP7 targets by iCLIP

| PRJNA388445 | ENA

Arabidopsis thaliana

Project description:Identification of BABY BOOM downstream targets

| PRJNA93529 | ENA

Gene expression from Inducible TOC1 expression in Arabidopsis seedlings

Project description:The first described feedback loop of the Arabidopsis circadian clock is based on reciprocal regulation between TOC1 and CCA1/LHY. CCA1 and LHY are MYB transcription factors that bind directly to the TOC1 promoter to negatively regulate its expression. Conversely, the activity of TOC1 has remained less well characterized. Genetic data supports that TOC1 is necessary for the reactivation of CCA1/LHY, but there is little description of its biochemical function. Here we show that TOC1 occupies specific genomic regions in the CCA1 and LHY promoters. Purified TOC1 binds directly to DNA through its CCT domain, which is similar to known DNA binding domains. Chemical induction and transient overexpression of TOC1 in Arabidopsis seedlings cause repression of CCA1/LHY expression demonstrating that TOC1 can repress direct targets, and mutation or deletion of the CCT domain prevents this repression showing that DNA binding is necessary for TOC1 action. Furthermore, we use the Gal4/UAS system in Arabidopsis to show that TOC1 acts as a general transcriptional repressor, and that repression activity is in the Pseudoreceiver (PR) domain of the protein. To identify the genes regulated by TOC1 on a genomic scale, we couple TOC1 chemical induction with microarray analysis and identify new potential TOC1 targets and output pathways. Together these results define the biochemical action of the core clock protein TOC1 and refine our perspective on how plant clocks function. Keywords: Expression profiling by array

2012-01-15 | GSE34897 | GEO

Arabidopsis thaliana

Project description:Identification of putative targets of AP3/PI

| PRJNA103607 | ENA

Arabidopsis thaliana

Project description:Identification of AtGRP7 targets by RIP-seq

| PRJNA388502 | ENA

Arabidopsis thaliana

Project description:Identification of LEAFY targets during reproductive transition

| PRJNA87931 | ENA

Genome-wide FLP/MYB88 binding sites in Arabidopsis thaliana

Project description:FLP and MYB88 are two paralogous MYB proteins, regulating the symmetric division of guard mother cell during Arabidopsis stomatal development. To understand their molecular functions, we performed genome-wide identification of FLP/MYB88 binding targets using ChIP-chip with FLP/MYB88 antibody. By comparing ChIP-chip between wild-type and flp-1 myb88 lines, a total genes were identified as putative direct targets for FLP/MYB88.

2010-09-01 | GSE19763 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data