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The medium-chain free fatty acid receptor GPR84 is expressed in pro-inflammatory macrophages and mediates inflammatory sequelae in endometriosis

ABSTRACT: The medium-chain free fatty acid receptor GPR84 is expressed in pro-inflammatory macrophages and mediates inflammatory sequelae in endometriosis

PROVIDER: PRJNA411952 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6066190_1.fastq.gz	Fastqsanger.gz
	SRR6066190_2.fastq.gz	Fastqsanger.gz
	SRR6066200_1.fastq.gz	Fastqsanger.gz
	SRR6066200_2.fastq.gz	Fastqsanger.gz
	SRR6066210_1.fastq.gz	Fastqsanger.gz

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The medium-chain free fatty acid receptor GPR84 is expressed in pro-inflammatory macrophages and mediates inflammatory sequelae in endometriosis

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The important role of GPR84 in MLL leukemogenesis

Project description:Beta-catenin signaling is required for establishment of leukemic stem cells (LSCs) in acute myeloid leukemia (AML), yet the upstream regulators that can augment this pathway are unknown. Through genome-wide gene expression analysis and functional studies, we identified an important role for GPR84 in MLL AML. Suppression of GPR84 significantly inhibited cell growth in pre-LSCs, reduced LSC frequency and impaired reconstitution of MLL AML. Furthermore, GPR84 conferred a growth advantage to Hoxa9/Meis1a transduced hematopoietic stem cells (HSCs). Our microarray analysis demonstrated that GPR84 overexpression significantly up-regulated a small set of MLL-fusion targets and beta-catenin co-effectors, and down-regulated a hematopoietic cell cycle inhibitor. These data thus reveal a previously unrecognized role of GPR84 in the maintenance of fully developed AML by sustaining aberrant beta-catenin signaling in LSCs. HSC-derived Hoxa9/Meis1a pre-LSCs were transduced with GPR84 cDNA or empty vector, and replated in methylcellulose supplemented with cytokines. Each group contains triplicate samples.

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Project description:We performed gene expression analysis human peritoneal endometriosis lesions, eutopic endometrium from endometriosis patients and peritoneum form endometriosis patients.The goal of the study was to analyse gene expression differences between peritoneal endometriosis lesion and eutopic endometrium and peritoneal endometriosis lesion and peritoneum.

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