Project description:We charactarize the transcriptomic response of Prunus persica to preventive treatment with PpPep1 and PpPep2 endogenous peptides. The most effective dose of each peptide was applied to leaves and we sequenced their transcriptomes after 1, 24 and 48 h using untreated plants as control. mRNA profiles were generated by deep sequencing, in triplicate, using Illumina HiSeq2000. Differential expression analysis was performed with R package DESeq2 version 1.20.0, genes were considered significant with FDR<0.01 and log2FC>1 or log2FC<-1. We identified a total of 2,076 genes exhibiting differential expression upon PpPep1 application and 1,985 for PpPep2, accounting for 8% of the P. persica genes, 6% in response to both peptides. The similarity of PpPep responses was higher shortly after treatment (1 h), with some asynchrony at later stages and some variation in the specific regulated genes, always within the same functional categories. qRT–PCR validation with 19 genes was performed using SYBR Green assays (R 0.92).
Project description:In the family Fagaceae, fertilization is delayed by several weeks to more than one year after pollination, leading to one- or two-year fruiting species depending on whether fruiting occurs in the same or the next year of flowering. Although delayed fertilization was recorded over a century ago, underlying mechanisms remain to be explored. To uncover the key genes associated with delayed fertilization, we obtain and analyze the comparative molecular phenology data over two years in one-year (Quercus glauca) and two-year fruiting species (Lithocarpus edulis).
Project description:Transcript profiling of leaves from Quercus ilex seedlings subjected to well-watering and drought-stress (irrigation withdrawal) conditions
Project description:We charactarize the regulated miRNAs of Prunus persica to preventive treatment of PpPep2 1 µM endogenous peptide. The peptide treatment was applied to leaves and we sequenced the miRNAs after 1 and 24 h using untreated plants as control. miRNA profiles were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500. Differential expression analysis of annotated pre_miRNA gene biotypes was performed with R package DESeq2 v1.26.0, considering significant epression values below FDR <0.05. We obtained 117,267,647 raw reads and we identified a total of 33 differential expressed miRNAs (DEM) belonging to 18 miRNA families upon PpPep2 application, accounting for 15% of the P. persica annotated miRNAs. miRNA regulation occurs principally one day after peptide treatment. The predicted mRNA targets and function of orthologous miRNAs are compatible with a regulation of PTI-related processes. Together with the transcriptomic response of P. persica to PpPep2 described at our previous RNA-Seq (Foix et al 2021), this suggests that miRNAs would have an active role in PTI regulation at these stages.