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Expression data from C57/Bl6 Wt mice CD4+ T cells after activation with anti-CD3 plus Wt APC or OX40L-Tg APC

ABSTRACT: Expression data from C57/Bl6 Wt mice CD4+ T cells after activation with anti-CD3 plus Wt APC or OX40L-Tg APC

PROVIDER: PRJNA413298 | ENA |

REPOSITORIES: ENA

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Expression data from C57/Bl6 Wt mice CD4+ T cells after activation with anti-CD3 plus Wt APC or OX40L-Tg APC

Project description:OX40 is a T cell costimulatory molecule in the tumor necrosis factor receptor (TNFR) superfamily. Unlike other costimulatory molecules, OX40 is not expressed by naive T cells, but its expression is rapidly induced following T cell activation, and OX40 costimulation plays a critical role in cell survival, proliferation, and generation of memory cells. We used microarrays to detail the global programme of gene expression of activated CD4 T cells underlying OX40 costimulation and identified distinct classes of up-regulated genes after OX40 costimulation.

2018-05-15 | GSE104611 | GEO

OX40L protects against inflammation-driven fibrosis

Project description:Fibrosis is a leading cause of deaths in industrialized countries and has no effective therapy. We demonstrated that blockade of OX40L prevented inflammation-driven fibrosis affecting the skin and the lungs and promotes regression of established dermal fibrosis in different murine models. To characterize the pathways involved in the protection of skin fibrosis and affected by OX40L blocking, we used microarrays and identified distinct genes differentially expressed between ox40l+/+ and ox40l-/- in the bleomycin-induced dermal fibrosis mouse model. Total RNA were extracted from lesional skin samples of 3 ox40l+/+ and 4 ox40l-/- male mice aged 9 weeks treated with bleomycin for 3 weeks, and were hybridized on Affymetrix microarrays.

2016-08-09 | E-GEOD-73705 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type APC and OX40L-TG APC induced T helper 17 cell Transcriptomes [RNA-seq]

Project description:Purpose:The goals of this study are to evaluate the effect of OX40 ligation on transcriptome profiling of in vitro polarized T helper 17 cell (RNA-seq). Methods: mRNA profiles of CD4+ T cells cultured in vitro under Th17-polarizing conditions for 48hrs (both Ctrl and OX40L treated groups) were generated by deep sequencing, using Illumina RapidRun. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) and TopHat followed by CuffDiff. qRT–PCR validation was performed using SYBR Green assays Results: Among the 398 genes that were significantly affected by OX40, only about 11% were previously identified as RORγt target genes in Th17 cells. Conclusions: Our study suggests that OX40 unlikely affects Th17 commitment, but most likely impairs their effector differentiation. As IL-17A and IL-17F are Th17-defining cytokines, their suppression suggests that OX40 may inhibits effector functions of Th17 cells.

2016-10-06 | GSE80010 | GEO

OX40L protects against inflammation-driven fibrosis

2016-08-09 | GSE73705 | GEO

RNA-seq analysis of mTOR{delta}APC and WT lung and spleen APC subsets

Project description:Purified CD11b+, Alveolar macrophage and HDM induced inflammatory DCs from WT and mTOR{delta} APC mice were profiled by RNA-seq to understand mTOR-dependent gene expression

2020-06-20 | GSE100316 | GEO

Apc-mutants act as supercompetitors in intestinal tumour initiation

Project description:Almost all colorectal cancers (CRC) present with mutations in the Apc gene, leading to unrestrained Wnt activation and the initiation of tumour development. We previously reported the competitive benefit of Apc-mutant intestinal stem cells (ISCs) within the crypt, however, the mechanism by which they outcompete their wild type (WT) neighbours remained elusive. Here, we studied the effect of Apc-mutants using an in vitro culture system of WT (Lgr5-CreErt2) and Apc-/- (Lgr5-CreErt2;Apcfl/fl) organoids . The expression patterns of WT and Apc-/- organoids revealed significant upregulation in specific secreted Wnt antagonists Notum, Wif1 and Dkk2. Furthermore, we studied the effect of the secreted antagonist by treating WT organoids with either WT or Apc-/- conditioned medium (CM) for 48 hours and observed a significant decrease in stem cell markers and an increase in goblet cell markers. We report that Apc-mutants act as bona fide supercompetitors by secreting Wnt antagonists that result in active differentiation of WT ISCs.

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Gene expression analysis of WT, Apc and ApcKras mouse intestine

Project description:Gene expression analysis of WT, Apc and ApcKras mouse intestine

| PRJNA673352 | ENA

Exosomal OX40L elicits positive T cell co-stimulation and promotes anti-tumor immunity through engagement of cytotoxic T lymphocytes

Project description:Exosomes play a role in immune regulation associated with cancer and other diseases. We generated exosomes that harbor the ligand of the co-stimulatory OX40 pathway, and demonstrated abundant levels of OX40L on the surface of exosomes, without inducing morphological and molecular alterations. Functionally, exosomal OX40L efficiently elicits positive co-stimulation of both human and murine T cells. The systemic administration of exosomal OX40L promotes anti-tumor immunity in an orthotopic melanoma mouse model, and the depletion of T cell subsets revealed that exosomal OX40L acts through CD8+ T cells. Mechanistically, exosomal OX40L enhances the proportion and the levels of cytotoxic markers in tumor-infiltrating CD8+ T lymphocytes. Taken together, using the OX40 pathway as proxy, we provide proof-of-principle evidence that the presence of positive co-stimulatory molecules on exosomes can functionally engage T cells to elicit anti-tumor immunity, paving the way to understand the biology of exosome-mediate T cell signaling and for further developing exosome-based reagents that engage T cell co-stimulation.

2025-02-10 | PXD031404 | Pride

Whole genome bisulfite genome analysis of OX40L-Jagged-1 expanded Treg and Teff cells

Project description:NOD mice were treated with PBS or OX40L and Jagged-1 proteins once a week for three weeks. A week after final treatment CD4+CD25- Teff cells and CD4+CD25hi Treg cells were sorted by FACS. Genomic DNA isolated from sorted cells and differences in DNA methylation pattern in these cell types between PBS and OX40L-Jagged-1 treated mice was analyzed by whole genome bi-sulfite sequencing.

2019-05-03 | GSE130617 | GEO

Synthetic Essentiality of Tryptophan 2,3-dioxygenase 2 in APC-Mutated Colorectal Cancer II

Project description:Comparative transcriptomic analysis of TAMs isolated from APC-WT and APC-KO MC38 orthotopic tumors express higher levels of multiple classical M2-like markers (CD163, CCL22, YM1) compared to APC-WT tumors after re-normalized the read counts of multiple M2 macrophage markers with housekeeping gene read counts (GAPDH and ACTB).

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