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Polycomb Repressive Complex 2 methylates Elongin A to regulate transcripiton [BrU-RNA-seq]

ABSTRACT: Polycomb Repressive Complex 2 methylates Elongin A to regulate transcripiton [BrU-RNA-seq]

PROVIDER: PRJNA413412 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6133700_1.fastq.gz	Fastqsanger.gz
	SRR6133700_2.fastq.gz	Fastqsanger.gz
	SRR6133701_1.fastq.gz	Fastqsanger.gz
	SRR6133701_2.fastq.gz	Fastqsanger.gz
	SRR6133702_1.fastq.gz	Fastqsanger.gz

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Polycomb Repressive Complex 2 methylates Elongin A to regulate transcription

Project description:Polycomb Repressive Complex 2 methylates Elongin A to regulate transcription

| PRJNA413407 | ENA

Polycomb Repressive Complex 2 methylates Elongin A to regulate transcripiton [BrU-RNA-seq]

Project description:Polycomb repressive complex 2 (PRC2-EZH2) methylates histone H3 at lysine 27 (H3K27) and is required to maintain gene repression during development. Misregulation of PRC2 is linked to a range of neoplastic malignancies, which is believed to involve methylation of H3K27. However, the full spectrum of non-histone substrates of PRC2 that might also contribute to PRC2 function is not known. We characterized the target recognition specificity of PRC2 and used the resultant data to screen for novel potential targets. The RNA polymerase II (Pol II) transcription factor, Elongin A (EloA), is methylated by PRC2 in vivo. Mutation of the methylated EloA residue decreased repression of many, but not all, PRC2 target genes as measured by both steady state and nascent RNA levels. We propose that PRC2 regulates transcription of a subset of target genes in part via methylation of EloA.

2017-11-16 | GSE104658 | GEO

Polycomb Repressive Complex 2 methylates Elongin A to regulate transcripiton [RNA-seq]

Project description:Polycomb Repressive Complex 2 methylates Elongin A to regulate transcripiton [RNA-seq]

| PRJNA413413 | ENA

Polycomb Repressive Complex 2 methylates Elongin A to regulate transcripiton [RNA-seq]

2017-11-16 | GSE104657 | GEO

Polycomb Repressive Complex 2 methylates Elongin A to tune transcript levels of targeted genes [ChIP-seq]

Project description:Polycomb repressive complex 2 (PRC2-EZH2) methylates histone H3 at lysine 27 (H3K27). PRC2 is required to maintain gene repression during development and differentiation and misregulation of PRC2 is linked to a range of neoplastic malignancies, activities that are believed to involve H3K27 methylation. The full spectrum of non-histone substrates of PRC2, however, is not known, and it is not known which other substrates might also contribute to the biological functions of PRC2. We characterized the target recognition specificity and substrate diversity of PRC2, and identified more than one hundred potential novel nuclear targets of PRC2. The RNA polymerase II (Pol II) transcription elongation factor, Elongin A (EloA), is an in vivo target of PRC2. Mutation of the EloA residue that is methylated by PRC2 decreases repression of numerous PRC2 target genes. We propose that this functional crosstalk between PRC2 and EloA tunes the level of repression of targeted genes and contributes to the biological functions of PRC2.

2017-11-16 | GSE104659 | GEO

Polycomb Repressive Complex 2 methylates Elongin A to tune transcript levels of targeted genes [ChIP-seq]

Project description:Polycomb Repressive Complex 2 methylates Elongin A to tune transcript levels of targeted genes [ChIP-seq]

| PRJNA413411 | ENA

Polycomb Repressive Complex 2 Methylates Elongin A to Regulate Transcription.

Project description:Polycomb repressive complex 2 (PRC2-EZH2) methylates histone H3 at lysine 27 (H3K27) and is required to maintain gene repression during development. Misregulation of PRC2 is linked to a range of neoplastic malignancies, which is believed to involve methylation of H3K27. However, the full spectrum of non-histone substrates of PRC2 that might also contribute to PRC2 function is not known. We characterized the target recognition specificity of the PRC2 active site and used the resultant data to screen for uncharacterized potential targets. The RNA polymerase II (Pol II) transcription elongation factor, Elongin A (EloA), is methylated by PRC2 in vivo. Mutation of the methylated EloA residue decreased repression of a subset of PRC2 target genes as measured by both steady-state and nascent RNA levels and perturbed embryonic stem cell differentiation. We propose that PRC2 modulates transcription of a subset of low expression target genes in part via methylation of EloA.

| S-EPMC5800316 | biostudies-literature

EPOP Functionally Links Elongin and Polycomb in Pluripotent Stem Cells

Project description:The cellular plasticity of pluripotent stem cells is thought to be sustained by genomic regions that display both active and repressive chromatin properties. These regions exhibit low levels of gene expression, yet the mechanisms controlling these levels remain unknown. Here, we describe Elongin BC as a binding factor at the promoters of bivalent sites. Biochemical and genome-wide analysis shows that Elongin BC is associated with Polycomb Repressive Complex 2 (PRC2) in pluripotent stem cells. Elongin BC is recruited to chromatin by the PRC2-associated factor EPOP (Elongin- and POlycomb-associated Protein, also termed C17orf96, esPRC2p48, E130012A19Rik), a protein expressed in the inner cell mass of the mouse blastocyst. Both EPOP and Elongin BC are required to maintain low levels of expression at PRC2 genomic targets. Our results indicate that keeping the balance between activating and repressive cues is a more general feature of chromatin in pluripotent stem cells than previously appreciated.

2016-11-21 | GSE79606 | GEO

Polycomb Repressive Complex 2 methylates Elongin A to regulate transcription

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-11-16 | GSE104660 | GEO

EPOP interacts with Elongin BC and USP7 to modulate the chromatin landscape (Elongin B KO)

Project description:Gene regulatory networks are pivotal for many biological processes. In mouse embryonic stem cells (mESCs) the transcriptional network can be divided into three functionally distinct modules: Polycomb, Core and Myc. The Polycomb module represses developmental genes, while the Myc module is associated with proliferative functions and its mis-regulation is linked to cancer development. Here, we show that in mESCs the Polycomb Repressive Complex 2 (PRC2) associated protein EPOP (a.k.a C17orf96, esPRC2p48, E130012A19Rik) co-localizes at chromatin with members of the Myc and Polycomb module. EPOP interacts with the transcription elongation factor Elongin BC and the H2B deubiquitinase USP7 to modulate transcriptional processes in mESCs similar to MYC. EPOP is commonly up-regulated in human cancer and we demonstrate that its loss impairs the proliferation of several human cancer cell lines. Our findings establish EPOP as a unique modulator of transcriptional processes, impacting both Polycomb and active gene transcription in mammalian cells.

2016-11-17 | GSE79318 | GEO

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