Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

OBESITY IS ASSOCIATED WITH IMPAIRED EXPRESSION OF THE GLYCOSYLTRANSFERASE EOGT IN DECIDUALIZING ENDOMETRIUM

ABSTRACT: OBESITY IS ASSOCIATED WITH IMPAIRED EXPRESSION OF THE GLYCOSYLTRANSFERASE EOGT IN DECIDUALIZING ENDOMETRIUM

PROVIDER: PRJNA413635 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6148310_1.fastq.gz	Fastqsanger.gz
	SRR6148310_2.fastq.gz	Fastqsanger.gz
	SRR6148311_1.fastq.gz	Fastqsanger.gz
	SRR6148311_2.fastq.gz	Fastqsanger.gz
	SRR6148312_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

OBESITY IS ASSOCIATED WITH IMPAIRED EXPRESSION OF THE GLYCOSYLTRANSFERASE EOGT IN DECIDUALIZING ENDOMETRIUM

Project description:In pregnancy, resistance of endometrial decidual cells to stress signals is critical for the integrity of the feto-maternal interface and, by extension, survival of the conceptus. O-GlcNAcylation is an essential post-translational modification that links glucose sensing to downstream stress resistance. Unexpectedly, decidualization of primary endometrial stromal cells (EnSCs) was associated with a 60% reduction in O-GlcNAc modified proteins, reflecting down-regulation of the enzyme that adds O-GlcNAc to substrates (O-GlcNAc transferase, OGT) but not the enzyme that removes the modification (O-GlcNAcase, OGA). Notably, EOGT, an endoplasmic reticulum-specific O-GlcNAc transferase that modifies a limited number of secreted and membrane proteins, was markedly induced in differentiating EnSCs. Knockdown of EOGT perturbed a network of decidual genes involved in multiple cellular functions. The most responsive gene downregulated upon EOGT knockdown in decidualizing cells was ENHO, which encodes adropin, a metabolic hormone involved in energy homeostasis and glucose and fatty acid metabolism. Analysis of mid-luteal endometrial biopsies revealed an inverse correlation between endometrial EOGT and ENHO expression and body mass index. Taken together, our findings reveal that obesity impairs the EOGT-adropin axis in decidual cells, which in turn provides a new mechanism that links between metabolic disorders to adverse pregnancy outcome.

2018-02-28 | GSE104721 | GEO

Transcriptomic exploration of decidualizing human endometrial stromal cell dynamics

2024-10-30 | GSE246591 | GEO

Epigenomic exploration of decidualizing human endometrial stromal cell dynamics

2024-10-30 | GSE246585 | GEO

Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cells migration

Project description:Glioblastoma (GBM) is the most aggressive primary brain tumor characterized by infiltrative migration of malignant glioma cells into the surrounding brain parenchyma. In this study, our analysis of GBM patient cohorts displayed significant higher expression of Glycosyltransferase 8 domain containing 1 (GLT8D1) compared to normal brain tissue and could be associated with impaired patient survival. Increased in vitro expression of GLT8D1 significantly enhanced migration of two different sphere-forming GBM cell lines. By in silico analysis we predicted the 3D-structure as well as the active site residues of GLT8D1. The introduction of point mutations in the predicted active site reduced its glycosyltransferase activity in vitro and consequently impaired GBM tumor cell migration. Examination of GLT8D1 interaction partners by LC-MS/MS implied proteins associated with cytoskeleton and intracellular transport as potential substrates. In conclusion, we demonstrated that the enzymatic activity of glycosyltransferase GLT8D1 promotes GBM cell migration.

2022-04-04 | PXD027854 | Pride

endometrium

Project description:Endometrium Gland - EPIC Methylation Array

| EGAD00010002199 | EGA

KrM-CM-<ppel-like Factor 9 and Progesterone Receptor Co-Regulation of Decidualizing Endometrial Stromal Cells: Implications for its Loss in the Pathogenesis of Endometriosis

Project description:Endometriosis is characterized by progesterone resistance and is associated with infertility. KrM-CM-<ppel-like Factor 9 (KLF9) is a progesterone receptor (PGR)-interacting protein, and mice null for Klf9 are subfertile. Whether loss of KLF9 contributes to progesterone resistance of eutopic endometrium of women with endometriosis is unclear. The aim of this study was to investigate KLF9 and PGR co-regulation of human endometrial stromal cell (HESC) transcriptome network. Microarray gene expression analysis was conducted in decidualizing HESC by silencing the expression of KLF9 and PGR, alone or in combination by a siRNA approach, to identify additional KLF9 and PGR co-regulated genes and signaling networks/pathways. HESC also treated with 8-bromo-cAMP, 17M-CM-^_-estradiol, and medroxyprogesterone acetate (cAME) to mimic stromal progression from a proliferative to a differentiated state.

2012-04-03 | E-GEOD-31683 | biostudies-arrayexpress

Analysis of chromatin accessibility in decidualizing human endometrial stromal cells

Project description:Spontaneous decidualization of the endometrium in response to progesterone signaling is confined to menstruating species, including humans and other higher primates. During this process, endometrial stromal cells (EnSCs) differentiate into specialized decidual cells that control embryo implantation. We subjected undifferentiated and decidualizing human EnSCs to Assay for Transposase Accessible Chromatin with sequencing (ATAC-seq) to map the underlying chromatin changes. A total of 185,084 open DNA loci were mapped accurately in EnSCs. Altered chromatin accessibility upon decidualization was strongly associated with differential gene expression. Analysis of 1,533 opening as well as closing chromatin regions revealed overrepresentation of DNA binding motifs for known decidual transcription factors (TFs) and identified putative new regulators. ATAC-seq footprint analysis provided evidence of TF binding at specific motifs. One of the largest footprints involved the most enriched motif, basic leucine zipper (bZIP), as part of a triple motif that also comprised the estrogen receptor- and Pax domain-binding sites. Without exception, triple motifs were located within Alu elements, suggesting a role for this primate-specific transposable element (TE) in the evolution of decidual genes. Although other TEs were generally underrepresented in open chromatin of undifferentiated EnSCs, several classes contributed to the regulatory DNA landscape that underpins decidual gene expression.

2018-02-28 | GSE104720 | GEO

Adipose stem cells from people with obesity show impaired mitochondrial and lysosomal functions

Project description:Adipose stem cells from people with obesity show impaired mitochondrial and lysosomal functions

| PRJNA918708 | ENA

PPARγ-dependent Remodeling of Translational Machinery in Adipose Progenitors is Impaired in Obesity

Project description:PPARγ-dependent Remodeling of Translational Machinery in Adipose Progenitors is Impaired in Obesity

| PRJNA1114106 | ENA

Transcriptomic exploration of decidualizing human endometrial stromal cell dynamics

Project description:Transcriptomic exploration of decidualizing human endometrial stromal cell dynamics

| PRJNA1033677 | ENA