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MicroRNAs and their targets engage in apple-Valsa mali interaction

ABSTRACT: microRNAs and their targets engage in apple-Valsa mali interaction

PROVIDER: PRJNA413773 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6153310.fastq.gz	Fastqsanger.gz
	SRR6153307.fastq.gz	Fastqsanger.gz
	SRR6153308.fastq.gz	Fastqsanger.gz
	SRR6153309.fastq.gz	Fastqsanger.gz

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microRNAs and their targets engage in apple-Valsa mali interaction

Project description:miRNAs were important regulators involving in plant-pathogen interactions. However, their roles in apple response to Valsa canker pathogen (Valsa mali, Vm) infection were poorly understood. In this study, we constructed two miRNA libraries using the bark tissues of apple twig (Malus domestica Borkh “Fuji”) inoculated with Vm (IVm) and PDA medium (control, BMd). Among the all miRNAs, 23 miRNAs were specifically isolated in BMd and 39 miRNAs were specifically isolated in IVm. Compared with BMd, the expression of 294 miRNAs decreased, and 172 miRNAs increased in IVm, respectively. We also identified the target genes of these miRNAs using degradome sequencing technology. In total, 353 differentially expressed miRNAs during the pathogen infection were detected to target 1 077 unigenes with 2 251 cleavage sites. Based on GO and KEGG analysis, the genes were found to be mainly related to transcription regulation and signal transduction. We further selected 17 miRNAs and 22 corresponding target genes to detect the expression profiles during pathogen infection. The results indicate that most of them are involved in apple twig-Vm interaction. What’s more, miRNAs and their corresponding target genes seem to regulate the apple twig-Vm interaction by forming many complicated regulation networks. It is worth that a conserved miRNAs mdm-miR482b, which was down regulated in IVm compared with BMd, has 14 potential target genes, and most of them were disease resistance related genes. More important, the feedback regulation of sRNA pathway in apple twig was much more complex and critical in the interaction between apple bark tissue and V. mali. The results provide insights into the crucial functions of miRNAs in the woody plant, apple tree-Vm interaction.

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LC-MSMS analysis of apple canker pathogen Valsa mali

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micro-like RNAs and their targets engage in DCL2-deleted Valsa mali

Project description:Dicer proteins are mainly responsible for generating small RNAs (sRNAs). In previous study, two DCL proteins in Valsa mali, the pathogenic fungus of apple tree Valsa canker, were found to be associated with the pathogenicity and generation of sRNAs. In this study, we constructed two milRNA libraries using the the wild-type 03-8 (MVm) and Vm-DCL2 deletion mutant (MD2) strains and the differential expression of miRNA-like RNAs (milRNAs) were analyzed based on the deep-sequencing of the wild-type and Vm-DCL2 mutant, respectively. In total, by comparing the expression difference of milRNAs between MVm and MD2, it was found that 33 milRNAs were not detected in MD2, 28 milRNAs were both detected in MVm and MD2. By statistical analysis, one and 17 milRNAs showed up-regulated and down-regulated expression in MD2 compared with MVm, respectively (p<0.05). Importantly, 10 milRNAs were not detected in MD2, and identified to be DCL2 dependent milRNAs. We also identified the target genes of these milRNAs using degradome sequencing technology and found they were associated with protein phosphatase, isocitrate dehydrogenase, phosphoglycerate mutase, etc. The study will lay a foundation for the comprehensive analysis of pathogenic mechanisms of V. mali, and enrich cognition of the generation and function of fungal sRNA.

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