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RNA-Seq analysis of localization along animal-vegetal axis of Xenopus laevis

ABSTRACT: RNA-Seq analysis of localization along animal-vegetal axis of Xenopus laevis

PROVIDER: PRJNA413987 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6160930_1.fastq.gz	Fastqsanger.gz
	SRR6160930_2.fastq.gz	Fastqsanger.gz
	SRR6160931_1.fastq.gz	Fastqsanger.gz
	SRR6160931_2.fastq.gz	Fastqsanger.gz
	SRR6160922_1.fastq.gz	Fastqsanger.gz

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RNA-Seq analysis of localization along animal-vegetal axis of Xenopus laevis

Project description:We combined cryosectining of oocytes along the animal-vegetal axis (first developmental axis) and RNA-Seq to determine localization profiles of coding and noncoding RNAs. It provides complete view on RNA localization. We found that nearly all RNAs are localized, but only small percentage is actively transported during oogenesis.

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Project description:In Xenopus laevis, a number of studies identified vegetal factors that specify the germ line, endoderm and dorsal axis, but there are few studies demonstrating roles for animal-enriched maternal mRNAs. Therefore, we carried out a microarray analysis to identify novel maternal transcripts enriched in animal blastomeres. We sought to maximize differences between animal and vegetal samples. To that end, we dissected 8-cell embryos into animal blastomeres and vegetal blastomeres, and further dissected the vegetal blastomeres into vegetal-most halves (VP) and equatorial regions (discarded).

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