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MRNA-seq in MCF7 cells in response to 4-hydroxytamoxifen (OHT)

ABSTRACT: mRNA-seq in MCF7 cells in response to 4-hydroxytamoxifen (OHT)

PROVIDER: PRJNA414078 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6163830.fastq.gz	Fastqsanger.gz
	SRR6163840.fastq.gz	Fastqsanger.gz
	SRR6163850.fastq.gz	Fastqsanger.gz
	SRR6163860.fastq.gz	Fastqsanger.gz
	SRR6163870.fastq.gz	Fastqsanger.gz

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mRNA-seq in MCF7 cells in response to 4-hydroxytamoxifen (OHT)

Project description:Purpose: Study the temporal effect of OHT on gene expression in MCF7 cells using mRNA-seq. Methods: MCF7 cells were treated with 100nM OHT for 2h, 6h and 24h or 24h with vehicle alone (ethanol). Six biological replicates of each time point were included in the analysis. For the library preparation the Illumina TruSeq Stranded mRNA Library Prep Kit High Throughput was used and 2 lanes of SE50 were run on HiSeq 4000. Results: We identify 27,520 genes of which 1,187 were significantly regulated in response to OHT in at least one time point. This included transcriptional repression of cell cycle genes as well as a number of known ERα target genes. Conclusions: We demonstrate a transcriptional response to OHT in MCF7 cells, with ERa target genes and genes linked to cell cycle progression being repressed.

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Transcriptional profile of human breast cancer MCF7 cells and clones thereof expressing human ERβ1 or ERβ2 upon treatment with antiestrogens and retinoids.

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Project description:Transcriptional profiling of 3D-retinas differentiated from mouse iPS cells comparing vehicle control- with 4-OHT-treated. 4-OHT is an inverse agonist of estrogen-related receptor beta (ERRÎ²), a rod-enriched transcription factor responsible for maintenance of rod photoreceptor cells and the treatment induces photoreceptor specific cell death in the 3D-retinas. Goal was to understand the mechanism of 4-OHT-induced degeneration of photoreceptor cells in the 3D-retinas. 4-OHT-induced gene expression in the 3D-retinas was measured at DD 26 when the photoreceptor cells were degenerated. Two-condition experiment, vehicle control- vs. 5 ÂµM 4-OHT-treated 3D-retinas. Biological replicates: each sample has 24 3D-retinas and 1 replicate.

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