Project description:Ssr4 was experimentally proven to be required for radial growth, aerial conidation, insect infection and virulence-related cellular events in the insect mycopathogen Beauveria bassiana. For in-depth insight into the essential role of Ssr4 in the insect mycopathogen, transcriptomic analysis was carried out via high throughput sequencing (RNA-Seq), resulting in nearly one fourth of the whole genome differentially expressed in the Dssr4 mutant versus wild-type strain.
Project description:Transcriptomic analysis of LaeA-deletion and overexpression LaeB in LaeA deletion strains in fungus Beauveria bassiana Examination of differential gene expressions by Beauveria bassiana wild type, LaeA-deletion and overexpression LaeB in LaeA deletion strains in fungus Beauveria bassiana
Project description:Insect pathogenic fungus Beauveria bassiana in one of the best studied insect biocontrol fungus, which infects insects by cuticle penetration. After breaking the cuticles, the fungus will propagate in insect hemocoel and kill insect hosts. It has also been found that the mycelia of B. bassiana can penetrate plant tissues to reach insect inside plant, e.g. corn borer (Ostrinia furnacalis), but do not cause damage to plants. The mechanism of fungal physiological plasticity is poorly understood. To accompany our genome sequencing work of B. bassiana strain ARSEF 2860, fungal transcriptional responses to different niches were studied using an Illumina RNA_seq technique. To examine fungal response to insect cuticle, conidia were inoculated on locust hind wings for 24 hours before used for RNA extraction. To evaluate fungal adaptation to insect hemocole, the fifth instar larvae of cotton bollworms were injected with spore suspension and fungal cells isolated by centrifugation in a step gradient buffer. To unveil the mechanism of interaction with plants, the fungus was grown in corn root exudates for 24 hours. After RNA sequencing, around three million tags were acquired for each sample and fungal transcriptional profiles were compared. Unveiling gene differential expression patterns when the insect biocontrol fungus Beauveria bassiana grown in insect hemocoel, corn root exudates and on insect cuticles.
Project description:Methods: Total RNAs were extracted from three 3-day-old hyphal cultures (replicates) of dim5 disruption and wild-type strains grown under normal culture conditions and and subjected to deep sequencing on Illumina Novaseq 6000 platform. The sequence reads that passed quality filters were mapped to the genome of Beauveria bassiana. All genes differentially expressed in the disruptant versus the wild-type strain were enriched to GO function classes and KEGG pathways. Results: The resultant transcriptome comprises 10915 genes annotated or not annotated. Among those, 1201 genes (genomic 11.59%) were dysregulated in the absence of dim5, including 489 down-regulated and 712 up-regulated. Conclusions: DIM5 has profound impact on the genomic expression of Beauveria bassiana.
Project description:BbTdp1 is a novel protein with the function of regulating conidium and blastospore yields, fungal morphological changes and pathogenicity in Beauveria bassiana.
