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MRNA Profiling of Murine 4T1 Breast Cancer Cells with Asns knocked down

ABSTRACT: mRNA Profiling of Murine 4T1 Breast Cancer Cells with Asns knocked down

PROVIDER: PRJNA414245 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6171333.fastq.gz	Fastqsanger.gz
	SRR6171334.fastq.gz	Fastqsanger.gz
	SRR6171335.fastq.gz	Fastqsanger.gz
	SRR6171336.fastq.gz	Fastqsanger.gz
	SRR6171337.fastq.gz	Fastqsanger.gz

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mRNA Profiling of Murine 4T1 Breast Cancer Cells with Asns knocked down

Project description:Murine 4T1-T Breast cancer cells were infected with retroviral vectors harboring shRNAs for murine or a Renilla, as negative control. After selection cells were harvested and processed with the NuGen Ovation RNAseq system V2. Libraries were sequenced on an Illumina platform and mapped using Bowtie2 to the mm10 genome prior to transcript quantification using HT-Count.

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Project description:Murine 4T1-T breast cancer cells were infected with retroviral vectors harboring shRNAs for murine or a Renilla, as negative control. Cells were orthotopically injected into NSG mice. After 19 days mice were euthanized and tumors and lungs were removed and digested into single cells. Cells were then placed in culture dishes with 6TG. Surviving cells were then harvested and processed with the NuGen Ovation RNAseq system V2. Libraries were sequenced on an Illumina platform and mapped using Bowtie2 to the mm10 genome prior to transcript quantification using HT-Count.

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mRNA Profiling of murine 4T1-T breast cancer cells with Asns knocked down after isolation from primary tumors and lung metastases with 6TG

Project description:mRNA Profiling of murine 4T1-T breast cancer cells with Asns knocked down after isolation from primary tumors and lung metastases with 6TG

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4T1 breast cancer cells: SPARC knock down vs. Control

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4T1 breast tumors: SPARC knock down primary tumors vs. Control primary tumors vs. Lung metastasis foci

Project description:Comparative analysis of the transcriptome of primary tumors generated from 4T1 cells transduced with a lentiviral vector expressing a siRNA against murine SPARC (4T1-C18), primary tumors generated from 4T1 cells transduced with a lentiviral vector expressing a scramble sequence (4T1-SCR) or lung metastasis foci from 4T1-SCR tumor-bearing mice (4T1-SCR MTTS). Three experimental conditions, 4T1-C18, 4T1-SCR and 4T1-SCR MTTS. Biological replicates: 4 4T1-C18, 4 4T1-SCR, 4 4T1-SCR MTTS independently grown in different mice. 2 days-old tumors and 30 days old lung foci. One replicate per array. All microarrays were processed the same day

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4T1 breast tumors: SPARC knock down primary tumors vs. Control primary tumors vs. Lung metastasis foci

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Differential expression levels of mRNAs in control 4T1 versus M2 macrophages co-cultured 4T1 murine breast cancer cells

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Gene expression of S100A10 knocked down human breast cancer cells

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