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Laticauda laticaudata

ABSTRACT: Laticauda laticaudata overview

PROVIDER: PRJNA414928 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	DRR003142.fastq.gz	Fastqsanger.gz
	DRR144984_1.fastq.gz	Fastqsanger.gz
	DRR144984_2.fastq.gz	Fastqsanger.gz
	DRR144985_1.fastq.gz	Fastqsanger.gz

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Project description:1. A neurotoxic protein similar to erabutoxins a and b of Laticauda semifasciata was isolated in crystalline form from the venoms of Laticauda laticaudata and Laticauda colubrina. The name ;laticotoxin a' is proposed. 2. Laticotoxin a is homogeneous by CM-cellulose column chromatography, disc electrophoresis and ultracentrifugation and by terminal amino acid analyses. 3. Laticotoxin a consists of 62 amino acid residues. The molecular weight by ultracentrifuging is 6520. 4. The minimal 50% lethal dose of laticotoxin a by intramuscular injection to mice is 0.13mug./g. body wt. The toxin attacks the postsynaptic membrane, competing with acetylcholine. 5. Radioactive amino acids are incorporated into laticotoxin a in vivo. The incorporation is inhibited by puromycin, suggesting that the biosynthesis of the toxin follows the mechanism of protein biosynthesis, although the toxin molecule is rather small as a protein.

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