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ABSTRACT: Preconditioning Biochar-Bacterial analyses-2

PROVIDER: PRJNA415285 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6201770.fastq.gz	Fastqsanger.gz
	SRR6201771.fastq.gz	Fastqsanger.gz
	SRR6201772.fastq.gz	Fastqsanger.gz
	SRR6201773.fastq.gz	Fastqsanger.gz
	SRR6201774.fastq.gz	Fastqsanger.gz

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Proteome analysis of bacterial biofilms in the biochar-based biofiltration systems

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Effect of biogas slurry combined with biochar on soil microbial function gene

Project description:The functional diversity of soil microbial communities was explored for a poplar plantation, which was treated solely with biogas slurry, or combined with biochar at different fertilization intensities over several years.

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Project description:Biochar soil bacterial sequencing data

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Project description:Ischemic stroke is a common acute CNS disorder leading to nearly half a million deaths per year in Europe. The high mortality is primarily owed to the limited treatment options of restoring blood flow in a narrow time window of several hours. Furthermore, inflammatory processes in the days and weeks after ischemic stroke contribute to tissue loss and neurological deficits. The key cells that influence and control this inflammatory cascade are microglia, the innate immune cells of the CNS. Microglia can be influenced and activated by e.g. lipopolysaccharide (LPS),a bacterial cell membrane component. It has been previously shown, that repetitive LPS stimuli prior to infarction (termed immunological preconditioning) lead to reduced infarct volumina in mouse models of ischemic stroke. Furthermore, our laboratory has shown, that phosphoinositide-3 kinase gamma mediates microglial functions after LPS-preconditioning. Hence, the aim of this work was to characterize proteomic alterations in microglia with (I) ischemic stroke in general in the tMCAO (transient middle cerebral artery occlusion) mouse model of ischemic stroke, (II) the influence of LPS-preconditioning on microglial proteomic alterations after tMCAO and (III) the role of PI3Ky in the microglial proteomic changes after tMCAO and preconditioning. This was done by a single LPS injection 3 days before tMCAO in wildtype mice and mice with PI3Ky knockout or knockin of the kinase dead form of PI3Ky.

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