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Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes [RNA-seq2]

ABSTRACT: Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes [RNA-seq2]

PROVIDER: PRJNA415304 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6201940_1.fastq.gz	Fastqsanger.gz
	SRR6201940_2.fastq.gz	Fastqsanger.gz
	SRR6201950_1.fastq.gz	Fastqsanger.gz
	SRR6201950_2.fastq.gz	Fastqsanger.gz
	SRR6201941_1.fastq.gz	Fastqsanger.gz

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Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes [RNA-seq2]

Project description:Here we were performed RNAseq to analyze the transcriptome changing of Hoxb5 in Pro/pre-B by over expression.

2018-02-07 | GSE105768 | GEO

Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes

Project description:Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes

| PRJNA414529 | ENA

Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes [ChIP-Seq]

Project description:Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes [ChIP-Seq]

| PRJNA414411 | ENA

Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes [ChIP-Seq]

Project description:Chipseq were performed to analyze the targets of Hoxb5 in pro-/pre-B by over expression.

2018-02-07 | GSE105007 | GEO

Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes [RNA-Seq]

Project description:Natural hematopoietic stem cells are currently the only cell resource to generate entire T lymphopoiesis in vivo. Altenatively, functional and non-tumorigenic T lymphocytes converted from the other cell types remain unsuccessful. Evidence is still lacking about the concept that 'lineage-switching factors' (LSFs) exist in HSC, whose re-activation in progeny cells can accomplish lineage conversions. Here we show that upon ectopic expression of Hoxb5(a HSC-specific transcription factor), the Pro-/Pre-B cells were induced into functional early T cell progenitors(iETPs) in vivo. The reprogramming process initiated in the bone marrow and accomplished in the thymus at ETP stage. Strikingly, the T lymphocytes derived from iETPs resemble their natural counterparts in transcriptome pattern, hierarchical differentiation, tissue distribution and immune functions. Our observations pave the way for achieving lineage regeneration by direct reprogramming autologous somatic cells in vivo. Further, our success in haematopoietic system may build a paradigm for LSF-mediated regeneration of other tissues.

2018-02-07 | GSE93107 | GEO

Hoxb5 reprograms murine multipotent blood progenitors into hematopoietic stem cell-like cells

Project description:We performed the single cell RNA-seq for the Hoxb5 Mac-1+CD48+ SK cells, bone marrow HSC, bone marrow MPP, and fetal liver HSC by Smart-Seq2.

2022-06-30 | GSE183800 | GEO

Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes.

Project description:Deletion of master regulators of the B cell lineage reprograms B cells into T cells. Here we found that the transcription factor Hoxb5, which is expressed in uncommitted hematopoietic progenitor cells but is not present in cells committed to the B cell or T cell lineage, was able to reprogram pro-pre-B cells into functional early T cell lineage progenitors. This reprogramming started in the bone marrow and was completed in the thymus and gave rise to T lymphocytes with transcriptomes, hierarchical differentiation, tissue distribution and immunological functions that closely resembled those of their natural counterparts. Hoxb5 repressed B cell 'master genes', activated regulators of T cells and regulated crucial chromatin modifiers in pro-pre-B cells and ultimately drove the B cell fate-to-T cell fate conversion. Our results provide a de novo paradigm for the generation of functional T cells through reprogramming in vivo.

| S-EPMC6190911 | biostudies-literature

Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-02-07 | GSE105057 | GEO

Hoxb5 reprograms murine multipotent blood progenitors into hematopoietic stem cell-like cells

Project description:Hoxb5 reprograms murine multipotent blood progenitors into hematopoietic stem cell-like cells

| PRJNA762032 | ENA

Neogenin-1 distinguishes between myeloid-biased and balanced Hoxb5+ mouse long-term hematopoietic stem cells

Project description:Hematopoietic stem cells (HSCs) self-renew and generate all blood cells. Recent studies with single-cell transplants and lineage tracing suggest that adult HSCs are diverse in their reconstitution and lineage potentials. However, prospective isolation of these subpopulations has remained challenging. Here, we identify Neogenin-1 (NEO1) as a unique surface marker on a fraction of mouse HSCs labeled with Hoxb5, a specific reporter of long-term HSCs (LT-HSCs). We show that NEO1+Hoxb5+ LT-HSCs expand with age and respond to myeloablative stress in young mice, while NEO1–Hoxb5+ LT-HSCs exhibit no significant change in number. Furthermore, NEO1+Hoxb5+ LT-HSCs are more often in the G2/S cell cycle phase compared to NEO1–Hoxb5+ LT-HSCs in both young and old bone marrow. Upon serial transplantation, NEO1+Hoxb5+ LT-HSCs exhibit myeloid-biased differentiation and reduced reconstitution, while NEO1–Hoxb5+ LT-HSCs are lineage-balanced and stably reconstitute recipients. Gene expression analysis reveals erythroid and myeloid priming in the NEO1+ fraction and association of quiescence and self-renewal-related transcription factors with NEO1− LT-HSCs. Finally, transplanted NEO1+Hoxb5+ LT-HSCs rarely generate NEO1–Hoxb5+ LT-HSCs, while NEO1–Hoxb5+ LT-HSCs repopulate both LT-HSC fractions. This supports a model in which dormant, balanced, NEO1–Hoxb5+ LT-HSCs can hierarchically precede active, myeloid-biased NEO1+Hoxb5+ LT-HSCs.

2019-11-10 | GSE130504 | GEO

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