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Uncultured bacteria

ABSTRACT: phytoplankton-associated bacteria responses to oil exposure

PROVIDER: PRJNA415472 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6205350_1.fastq.gz	Fastqsanger.gz
	SRR6205350_2.fastq.gz	Fastqsanger.gz
	SRR6205360_1.fastq.gz	Fastqsanger.gz
	SRR6205360_2.fastq.gz	Fastqsanger.gz
	SRR6205370_1.fastq.gz	Fastqsanger.gz

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Mucosal responses of healthy humans to exponentially growing or stationary Lactobacillus plantarum bacteria

Project description:Some commensal bacteria stimulate the immune system but do not present specific antigenicity. Such adjuvant effects have been reported for the bacterial species Lactobacillus plantarum. To study in vivo human responses to L. plantarum, a randomised double-blind placebo-controlled cross-over study was performed. Healthy adults were provided preparations of living and heat-killed L. plantarum bacteria, biopsies were taken from the intestinal mucosa and altered transcriptional profiles were analysed. Transcriptional profiles of human epithelia displayed striking differences upon exposure to living L. plantarum bacteria harvested at different growth phases. Modulation of NF-κB-dependent pathways was central among the major altered cellular responses. This unique in vivo study shows which cellular pathways are associated with the induction of immune tolerance in mucosal tissues towards common adjuvanticity possessing lactobacilli. Keywords: mucosal response of healthy adult humans to lactic acid bacteria

2009-01-21 | GSE11355 | GEO

Mucosal responses of healthy humans to exponentially growing or stationary Lactobacillus plantarum bacteria

2009-01-20 | E-GEOD-11355 | biostudies-arrayexpress

Transcriptomic responses to Deepwater Horizon oil in mahi-mahi (Coryphaena hippurus) embryos reveals time and oil-dependent linkages between molecular initiating events and developmental toxicity

Project description:Purpose:To help identify molecular mechanisms and pathways potentially involved in the developmental toxicity for fish exposed to Deepwater Horizon (DWH) oil, transcriptomic profiles in mahi-mahi (Coryphaena hippurus) embryos exposed to different DWH oils (source and artificially weathered oil) were evaluated at different critical windows of development using High Throughput Sequencing (HTS). Methods:Total mRNA profiles of 24, 48, 96 hpf mahi-mahi larvae after slick and source oil exposure were generated by deep sequencing, in triplicate, using Illumina HiSeq2500. qRT–PCR validation was performed using SYBR Green assays. Results: Exposure to slick oil induced more pronounced changes in gene expression over time than did exposure to source oil. Predominant transcriptomic responses included alteration of E1F2 signaling, steroid biosynthesis, ribosome biogenesis, perturbation in eye development and peripheral nervous, and activation of P450 pathway. Comparisons of changes of cardiac / Ca2+-associated genes with phenotypic responses revealed reduced heart rate and increased pericardial edema in larvae exposed to slick oil but not source oil.

2016-07-07 | GSE79675 | GEO

Transcriptomic responses to Deepwater Horizon oil in mahi-mahi (Coryphaena hippurus) embryos reveals time and oil-dependent linkages between molecular initiating events and developmental toxicity

Project description:Purpose:To help identify molecular mechanisms and pathways potentially involved in the developmental toxicity for fish exposed to Deepwater Horizon (DWH) oil, transcriptomic profiles in mahi-mahi (Coryphaena hippurus) embryos exposed to different DWH oils (source and artificially weathered oil) were evaluated at different critical windows of development using High Throughput Sequencing (HTS). Methods:Total mRNA profiles of 24, 48, 96 hpf mahi-mahi larvae after slick and source oil exposure were generated by deep sequencing, in triplicate, using Illumina HiSeq2500. qRTâPCR validation was performed using SYBR Green assays. Results: Exposure to slick oil induced more pronounced changes in gene expression over time than did exposure to source oil. Predominant transcriptomic responses included alteration of E1F2 signaling, steroid biosynthesis, ribosome biogenesis, perturbation in eye development and peripheral nervous, and activation of P450 pathway. Comparisons of changes of cardiac / Ca2+-associated genes with phenotypic responses revealed reduced heart rate and increased pericardial edema in larvae exposed to slick oil but not source oil. Total mRNA profiles of 24, 48, 96 hpf mahi-mahi larvae after slick and source (mass) oil exposure were generated by deep sequencing, in triplicate, using Illumina HiSeq2500.

2016-07-07 | E-GEOD-79675 | biostudies-arrayexpress

uncultured candidate bacteria

Project description:uncultured candidate bacteria Raw sequence reads

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uncultured freshwater bacteria

Project description:uncultured freshwater bacteria Raw sequence reads

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Interaction and Signaling Between a Cosmopolitan Phytoplankton and Associated Bacteria

Project description:Interaction and Signaling Between a Cosmopolitan Phytoplankton and Associated Bacteria

| PRJNA273418 | ENA

Project description:Marine bacteria associated with phytoplankton

| PRJNA1089792 | ENA

uncultured bacterium

Project description:Dinoflagellate-associated bacteria metagenomics

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Genome-wide transcriptional responses to Deepwater Horizon oil in red drum (Sciaenops ocellatus) embryos and larvae

Project description:Purpose:To investigate the transcriptomic profiles in red drum embryos reflective of the DWH oil toxicity at different critical windows of development and to predict the most impacted biological processes and pathways based on differentially expressed gene transcripts at different developmental stages using High Throughput Sequencing (HTS). Methods:Total mRNA profiles of 24, 48, 72 hpf red drum larvae after slick and source oil exposure were generated by deep sequencing, in triplicate, using Illumina HiSeq2500. Results:Oil type-dependent transcriptional effects were observed, with more significant by source oil exposure at 24 and 48 h, and similar responses by source and slick at 72 hpf. Informatic analyses indicated source oil exposure started significant perturbation in metabolism, AhR, visual, and cardiac-associated genes as early as 24 hpf. Both source and slick oil significantly affected EIF2 pathway, nervous and cardiovascular systems from 48 hpf to 72 hpf.

2017-11-14 | GSE90113 | GEO

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