Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Nascent-seq data from DRB-treated/untreated human U2OS cell line

ABSTRACT: Nascent-seq data from DRB-treated/untreated human U2OS cell line

PROVIDER: PRJNA415484 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6205340.fastq.gz	Fastqsanger.gz
	SRR6205341.fastq.gz	Fastqsanger.gz
	SRR6205332.fastq.gz	Fastqsanger.gz
	SRR6205342.fastq.gz	Fastqsanger.gz
	SRR6205333.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Nascent-seq data from DRB-treated/untreated human U2OS cell line

Project description:Confluent U2OS cells were released to serum-free medium, and continued to grow for 36 h (Per2-dLuc peak) or 48 h (Per2-dLuc trough), then added DRB (10uM) treatment for 0.5, 1.5, 3.5 h, then added BrU (250uM) for 0.5 h, harvested total RNA, captured with anti-BrU antibody, and conduced hi-seq.

2020-10-23 | GSE105815 | GEO

Chem-seq data from 5'-biotin-DRB-labelled human U2OS cells

Project description:Confluent U2OS cells were released to serum-free medium, and continued to grow for 36 h (Per2-dLuc peak), then added biotin-DRB (40uM) treatment for 0.5, then fixed the cells, harvested genomic DNA by Strepavidin agarose beads, and conducted hi-seq sequencing

2022-10-25 | GSE106193 | GEO

Mapping of nascent RNA upon release of DRB in WT and KD of RECQL5

Project description:Global Run-On has been performed on WT or KD for RECQL5 cells after release from DRB. When RECQL5 is knocked-down the transcriptional wave front is more advanced, suggesting that transcription is faster. Constitutive knock-down cell lines expressing or not endogenous levels of shRNA resistant RECQL5 under a Doxycycline inducible promoter were treated with high doses of DRB to block transcription. Upon release into fresh medium we were able to follow how much and how fast the RNA Pol II progresses through genes by mapping nascent RNA by Run-On. The experiment was performed in two cell line clones.

2014-06-09 | E-GEOD-49133 | biostudies-arrayexpress

Chem-seq data from 5'-biotin-DRB-labelled human U2OS cells

Project description:Chem-seq data from 5'-biotin-DRB-labelled human U2OS cells

| PRJNA415843 | ENA

U2OS-ERa, U2OS-ERb, and U2OS-ERab cells treated with 4HT or E2

Project description:U2OS-ERa, U2OS-ERb, and U2OS-ERab cells treated with 4HT or E2 Keywords: other

2005-04-06 | GSE2292 | GEO

RNA-seq of U2OS treated with Harmine or INDY and their controls

Project description:RNA-seq was applied for identifying the change of transcriptome between U2OS cells with starvation-induced quiescence treated with Harmine Hydrochloride or INDY and the untreated controls.

2023-02-01 | GSE168401 | GEO

Whole-genome CRISPR screen of etoposide-treated U2OS and U2OS p53KO cells, and RNAseq of U2OS cells incubated in media conditioned by etoposide-treated U2OS or U2OS p53KO cells.

Project description:CRISPR screen: U2OS or U2OS p53KO cells expressing Cas9 were transduced with a whole-genome library of CRISPR sgRNAs, then treated with either DMSO or etoposide. Differential sgRNA abundances were calculated for each condition and used to determine the effect of each single gene knockout on fitness and the drug-induced death rate. RNA-Seq: U2OS or U2OS p53KO cells were cultured with either DMSO or etoposide for 48 hours, and then U2OS cells were incubated in this conditioned media for 8 hours. RNA was collected and use to observe differential expression changes between conditions.

2023-05-17 | GSE232592 | GEO

Mapping of nascent RNA upon release of DRB in WT and KD of RECQL5

2014-06-09 | GSE49133 | GEO

The response of A549 transcriptome to RNA Pol II slowdown with alpha-amanitin and DRB

Project description:According to current estimates, more than 40% of nascent introns are spliced co-transcriptionally and, consequently, the speed of RNA polymerase II elongation must fundamentally affect the rate of RNA folding, spliceosome assembly, and the resulting mature transcript. RNA-Seq data was generated using RNAPII elongation inhibitors DRB and a-amanitin in A549 cells and used to look at genome-wide splicing changes

2020-06-27 | GSE153303 | GEO

Transcriptomics profile of polyQ expressing U2OS cells and clofazimine treated polyQ expressing U2OS cells

Project description:To better understand the mechanism by which CFZ impacts polyQ toxicity, we conducted a transcriptomic analysis in polyQ94-EGFP expressing U2OS (5μM, 8 days). Data from a transcriptomics experiment in polyQ94-U2OS cells treated with CFZ (5 μM, 8day) was compared to a DMSO-treated control.

2023-05-11 | GSE222758 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data