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Transcriptome data from ascorbic acid 2-glucose treated human or mouse embryonic stem cells

ABSTRACT: Transcriptome data from ascorbic acid 2-glucose treated human or mouse embryonic stem cells

PROVIDER: PRJNA415820 | ENA |

REPOSITORIES: ENA

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Transcriptome data from ascorbic acid 2-glucose (AA2G) treated human embryonic stem cell vs. non treated human embryonic stem cell

Project description:Transcriptome data from ascorbic acid 2-glucose (AA2G) treated human embryonic stem cell vs. non treated human embryonic stem cell

| PRJNA415829 | ENA

Transcriptome data from ascorbic acid 2-glucose (AA2G) treated murine embryonic stem cell vs. non treated murine embryonic stem cell

Project description:Transcriptome data from ascorbic acid 2-glucose (AA2G) treated murine embryonic stem cell vs. non treated murine embryonic stem cell

| PRJNA415830 | ENA

Transcriptome data from ascorbic acid 2-glucose (AA2G) treated murine embryonic stem cell vs. non treated murine embryonic stem cell

Project description:Naive or primitive states of stem cells (SCs) resided in specific niches are unstable and difficult to stabilized in vitro. Vitamin-C (VitC), more than suppressing oxygen radicals, exerts the pleiotropic effects on primitive SC functions through various mechanisms. However, instability and unfavorable cellular toxicity by liable oxidation present crucial pitfalls in its reliable application. Here, we show that a VitC derivate, ascorbic acid 2-glucoside (AA2G), without cellular toxicity, stably induces naïve or primitive status of embryonic (ESCs), inducible pluripotent (iPSCs), and mesenchymal SCs (MSCs). AA2G supplement recapitulated the well-known mechanisms of VitC including promoting TET dependent DNA demethylation in murine ESCs or suppressing p53 in iPSCs generation. Particularly, activation of cAMP responsive element binding protein-1 (CREB1) pathway was commonly involved in AA2G potency of ESCs, iPSCs, and MSCs. Importantly, MSCs maintained with AA2G supplement increased the therapeutic outcomes in an asthma mouse model by enhancing their self-renewal, anti-inflammatory, and engraftment capacity. Thus, this study demonstrates that AA2G supplement should be of broad utility to provide an environment supporting naïve pluripotent or primitive state of several types of SCs which critically influences their developmental potency and also efficacy of therapeutic applications.

2019-11-18 | GSE106184 | GEO

Transcriptome data from ascorbic acid 2-glucose (AA2G) treated human embryonic stem cell vs. non treated human embryonic stem cell

2019-11-18 | GSE106183 | GEO

Transcription profiling of human skin fibroblasts treated with ascorbic acid

Project description:Effect of Ascorbic acid on human genome

2009-10-15 | E-MEXP-1861 | biostudies-arrayexpress

Ascorbic acid induces transient global active chromatin remodelling, providing clues for its anti-cancer effect

Project description:In addition to the role of antioxidant, ascorbic acid (reducing Vitamin C) is an important cofactor for Fe2+ and α-ketoglutarate (α-KG) dependent dioxygenases (Fe2+/α-KGDDs) that comprise many diverse enzymes, including collagen prolyl hydroxylases, jmjC (Jumonji C) domain containing histone demethylases, Ten-eleven translocation (TET) 5-methyl cytosine (5mC) dioxygenases, and N6-methyl adenosine (m6A) demethylase FTO and ALKBH5. Ascorbic acid was reported to induce global epigenetic reprogramming. Here we optimized the library construction flow chart of single-stranded DNA profiling method KAS-seq and utilized KAS-seq to profile transient chromatin states changes upon ascorbic acid treatment for 10 min. We identified several critical pathways affected by ascorbic acid treatment, providing some clues for explaining the reported positive impact of anti-cancer, anti-depression, and anti-obesity for taking ascorbic acid.

2023-07-25 | GSE237843 | GEO

Transcriptional response of Streptococcus pneumoniae D39 to ascorbic acid

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in M17 medium to grown in M17 medium + 10mM ascorbic acid (AM17).

2015-01-21 | GSE64105 | GEO

Ascorbic acid-dependent gene expression in Streptococcus pneumoniae and the activator function of the transcriptional regulator UlaR2.

Project description:In this study, we have explored the impact of ascorbic acid on the transcriptome of Streptococcus pneumoniae D39. The expression of several genes and operons, including the ula operon (which has been previously shown to be involved in ascorbic acid utilization), the AdcR regulon (which has been previously shown to be involved in zinc transport and virulence) and a PTS operon (which we denote here as ula2 operon) were altered in the presence of ascorbic acid. The ula2 operon consists of five genes, including the transcriptional activator ulaR2. Our β-galactosidase assay data and transcriptome comparison of the ulaR2 mutant with the wild-type demonstrated that the transcriptional activator UlaR2 in the presence of ascorbic acid activates the expression of the ula2 operon. We further predict a 16-bp regulatory site (5’-ATATTGTGCTCAAATA-3’) for UlaR2 binding in the Pula2. Furthermore, we have explored the effect of ascorbic acid on the expression of the AdcR regulon. Our ICP-MS analysis showed that addition of ascorbic acid to the medium causes zinc starvation in the cell that leads to the activation of the AdcR regulon. This SuperSeries is composed of the SubSeries listed below.

2015-01-21 | GSE64107 | GEO

Transcriptome analysis of the effects of GSK-J4 and L-ascorbic acid in female embryonic stem cells

Project description:The effects of a histone demethylase inhibitor, GSK-J4, and L-ascorbic acid for the transcriptome in female ES cells were analyzed by RNA-sequence. Total RNA was used for high-throughput sequence with Illumina HiSeq 2500 and mapped to mm10.

2015-05-08 | GSE67674 | GEO

Ascorbic acid reprogrames epigenome and epitranscriptome by recycling Fe (III) into Fe (II) in the catalytic cycle of dioxygenases (m6A)

Project description:Ascorbic acid has been reported to stimulate DNA iterative oxidase TET enzymes, Jumonji C-domain-containinghistone demethylase and potentially RNA m6A demethylase FTO and ALKBH5 as a cofactor. Although ascorbic acid has been widely investigated in reprogramming DNA and histone methylation status in vitro, in cell lines and mouse models, its specific role in the catalytic cycle of dioxygenases remains enigmatic. Here we systematically investigated the stimulation of ascorbic towards TET2, ALKBH3, histone demethylases and FTO. We find that ascorbic acid reprograms epitranscrip-tome by erasing the hypermethylated m6A sites. Biochemistry and Electron spin resonance (ESR) assays demonstrate that ascorbic acid enters the active pocket of dioxygenases, reduces Fe (III), either incorporated upon protein synthesis or generated upon rebounding the hydroxyl radical during oxidation, into Fe (II). Finally, we propose a new model for the catalytic cycle of dioxygenases by adding in the essential dynamic cofactor, ascorbic acid. Ascorbic acid refreshes and regenerates inactive dioxygenase through recycling Fe (III) into Fe (II) in a dynamic “hit-and-run” manner.

2024-02-01 | GSE242758 | GEO

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