Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Facilitates Quantitative Analysis of allele specific transcription of PVT1 and MYC in mouse nueronal progenitor clonal cells

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of allele specific transcription of PVT1 and MYC in mouse nueronal progenitor clonal cells

PROVIDER: PRJNA415860 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6219430_1.fastq.gz	Fastqsanger.gz
	SRR6219430_2.fastq.gz	Fastqsanger.gz
	SRR6219440_1.fastq.gz	Fastqsanger.gz
	SRR6219440_2.fastq.gz	Fastqsanger.gz
	SRR6219450_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 216

Similar Datasets

Next Generation Sequencing Facilitates Quantitative Analysis of allele specific transcription of PVT1 and MYC in mouse nueronal progenitor clonal cells

Project description:PVT1 and MYC transcription is inversely correlated

2018-05-31 | GSE106197 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of chromatin conformational interaction between MYC and PVT1 in MCF7

Project description:MYC-PVT1 interaction is cell line specific

2018-05-31 | GSE106153 | GEO

Allele specific expression showing the cis-regulation of Pvt1 and Myc in mouse

Project description:Allele specific expression showing the cis-regulation of Pvt1 and Myc in mouse

| PRJNA415858 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of chromatin conformational interaction between MYC and PVT1 in MDA-MB-231, MCF7, HCT116 cancer cell line

Project description:MYC-PVT1 interaction is cell line specific

2018-05-31 | GSE97588 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis for H3K27ac mediated chromatin conformation capture of Wild Type and PVT1 Knockdown by CRISPRi in MDA-MB-231 human breast cancer cell line

Project description:CRISPRi targeting PVT1 specifically decrease PVT1-MYC interaction

2018-05-31 | GSE97585 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of chromatin conformational interaction between MYC and PVT1 in MCF7

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of chromatin conformational interaction between MYC and PVT1 in MCF7

| PRJNA415772 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and PVT1 Knockdown by CRISPRi in MDA-MB-231 human breast cancer cell line

Project description:Induced MYC expression by CRISPRi targeting PVT1 was validated by RNA-seq

2018-05-31 | GSE97587 | GEO

N6-methyladenosine Modification of lncRNA Pvt1 Governs Epidermal Stemness

Project description:Dynamic chemical modifications of RNA represent novel and fundamental mechanisms that regulate stemness and tissue homeostasis. Rejuvenation and wound repair of mammalian skin are sustained by epidermal progenitor cells, which are localized within the basal layer of the skin epidermis. N6-methyladenosine (m6A) is one of the most abundant modifications found in eukaryotic mRNA and lncRNA (long non-coding RNA). In this report, we survey changes of m6A RNA methylomes upon epidermal differentiation, and identify Pvt1, a lncRNA whose m6A modification is critically involved in sustaining stemness of epidermal progenitor cells. With genome-editing and a mouse genetics approach, we show that ablation of m6A methyltransferase or Pvt1 impairs the self-renewal and wound healing capability of skin. Mechanistically, methylation of Pvt1 transcripts enhances its interaction with MYC and stabilizes the MYC protein in epidermal progenitor cells. Our study presents a global view of epitranscriptomic dynamics that occur during epidermal differentiation and identifies the m6A modification of Pvt1 as a key signaling event involved in skin tissue homeostasis and wound repair.

2021-02-01 | GSE165912 | GEO

A renal cancer-associated, renal tubule-specific, HIF-binding enhancer of oncogenic MYC and PVT1 expression

Project description:Clear cell renal cell carcinoma is characterized by loss of function of the von Hippel-Lindau tumor suppressor gene (VHL) and unrestrained activation of hypoxia inducible transcription factors (HIF). Genetic and epigenetic determinants can impact on HIF pathways. A recent genome-wide association study identified single nucleotide polymorphisms (SNPs) in an intergenic region on chromosome 8 that modify the risk of developing renal cancer. The SNPs are located in a putative regulatory region between the oncogenes MYC and PVT1. Using capture C assay and genome editing we show that HIF-binding to this regulatory element is necessary to trans-activate MYC and PVT1 expression specifically in cells of renal tubular origins. Moreover, we demonstrate that the risk associated polymorphisms increase chromatin accessibility and activity as well as binding of HIF to the enhancer. These findings further strengthen the hypothesis that genetic variation at HIF-binding sites modulates the oncogenic transcriptional output of the VHL-HIF axis and provide a functional explanation for disease associated effects of SNPs in ccRCC.

2017-02-12 | GSE84444 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of PVT1 overexpressed MDA-MB-231 cells.

Project description:PVT1 can regulates glycolysis related genes expression.

2024-05-01 | GSE175736 | GEO