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Rainbow-seq: combining cell division lineage tracking with single-cell RNA sequencing for understanding cell fate decision in preimplantation development

ABSTRACT: Rainbow-seq: combining cell division lineage tracking with single-cell RNA sequencing for understanding cell fate decision in preimplantation development

PROVIDER: PRJNA416125 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6230600_1.fastq.gz	Fastqsanger.gz
	SRR6230600_2.fastq.gz	Fastqsanger.gz
	SRR6230610_1.fastq.gz	Fastqsanger.gz
	SRR6230610_2.fastq.gz	Fastqsanger.gz
	SRR6230620_1.fastq.gz	Fastqsanger.gz

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Rainbow-seq: combining cell division lineage tracking with single-cell RNA sequencing for understanding cell fate decision in preimplantation development

Project description:Our main question is whether there are reproducible cell-to-cell transcriptome differences in 4-cell embryos that are attributable to their different progenitor cells at the 2-cell stage. If the answer is negative, we wish to test whether there are reproducible cell-to-cell transcriptome differences in 8-cell embryos that are attributable to their different progenitor cells at the 2-cell stage. To address these questions, we developed the Rainbow-seq method that uses fluorescent protein genes to trace cell divisions in preimplantation embryonic development (PED) embryos

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