ABSTRACT: Metatranscriptome of Cenococcum geophilum from Champenoux, France - Poplar tremula x Poplar alba 717-1B4 mycorrhiza - Myc Cg1.58_Poplar_earlycontact_3Rb metatranscriptome
Project description:The Poplar transcriptome was analyzed in mycorrhizal root tips in contact with Laccaria bicolor for 2 weeks. During mycorrhization the roots were treated with either 250M-BM-5m ACC, 10nM JA or 500M-BM-5M SA and compared to untreated mycorrhiza or control roots without contact to L. bicolor. In addition the poplar mutants 35S::PttACO1 and 35S::Atetr1 were used We performed 27 hybridizations (NimbleGen) with samples derived from Populus tremula x Populus alba clone 717-1B4 control roots, untreated mycorrhiza, SA-treated mycorrhiza, ACC-treated mycorrhiza and JA-treated mycorrhiza (3 biological replicates each) as well as Populus tremula x Populus tremuloides T89 control roots, mycorrhiza, 35S::PttACO1 mycorrhiza and 35S::Atetr1-1 mycorrhiza (3 biological replicates). All samples were labeled with Cy3.
Project description:Metatranscriptome of Poplar tremula x Poplar alba 717-1B4 inoculated with Cenococcum geophilum - Myc Cg1.58_Poplar_latecontact_1R metatranscriptome
Project description:Metatranscriptome of Poplar tremula x Poplar alba 717-1B4 inoculated with Cenococcum geophilum - Myc Cg1.58_Poplar_earlycontact_2R metatranscriptome
Project description:Metatranscriptome of Poplar tremula x Poplar alba 717-1B4 inoculated with Cenococcum geophilum - Myc Cg1.58_Poplar_latecontact_2R metatranscriptome
Project description:Metatranscriptome of Poplar tremula x Poplar alba 717-1B4 inoculated with Cenococcum geophilum - Myc Cg1.58_Poplar_latecontact_3R metatranscriptome
Project description:Metatranscriptome of Poplar tremula x Poplar alba 717-1B4 inoculated with Cenococcum geophilum - Myc Cg1.58_Poplar_earlycontact_1R metatranscriptome
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Cenococcum geophilum ectomycorrhizal poplar roots compared to free-living mycelium . Ectomycorrhizal poplar roots and control mycelium were harvested after 60 days and used for RNA extraction. Reads of 150bp were generated and aligned to the C. geophilum reference genome (https://genome.jgi.doe.gov/Cenge3/Cenge3.home.html).
Project description:The Poplar transcriptome was analyzed in Populus tremulaxPopulus alba clone 717-1B4 control roots and in two poplar lines overexpressing MiSSP7.
Project description:Illumina technology was used to generate mRNA profiles of galls from root-knot nematodes infected and corresponding uninfected roots from Poplar CAD and WT lines. RNA was extracted from 3 replicates.TruSeq mRNA Stranded libraries were constructed and after pooling and normalization of libraries, sequencing was done on a NextSeq500 Sequencing System. Raw reads were trimmed for quality and mapped to the substituted genome sequence of P. tremula x P. alba 717-1B4 using CLC Genomics Workbench v9.5.2 and the primary transcripts only.
Project description:Illumina technology was used to generate mRNA profiles of Populus tremula x alba 717-1B4 control roots and Laccaria bicolor S238N ectomycorrhiza. Total RNA was extracted, TruSeq mRNA Stranded libraries were constructed and and sequenced (2 x 150 bp Illumina HiSeq3000) at the Genotoul sequencing facilities (Toulouse, France). Raw reads were trimmed for low quality (quality score 0.05), Illumina adapters and sequences shorter than 15 nucleotides and aligned to the Populus trichocarpa v4.1 primary transcripts available at Phytozome (https://phytozome-next.jgi.doe.gov/info/Ptrichocarpa_v4_1l) using CLC Genomics Workbench v24.