Project description:To identify putative miRNA targets for uc.372, we overexpressed uc.372 in HepG2 cells and carried out a microarray analysis. A total of 66 miRNAs present in the array demonstrated 1.5-fold upregulation or downregulation of expression upon uc.372 transfection.
Project description:MicroRNAs are important cellular regulators and their dysfunctions are associated with various disease. miR-371/372/373 was found co-regulated in HBV-producing HepG2.2.15 cells when compared to its non-HBV producing maternal HepG2 cells. To obtain a glimpse of the potential influence of the enforced miR-371-372-373 cluster in HepG2 gene expression, a two-color Capitalbio 70-mer oligo microarray platform, which contained 21,329 well-characterized human gene probes, was used to identify the differentially expressed genes between miR-371-372-373-HepG2 and mock-HepG2 in two independent biological replicate. miR-371-372-373-HepG2 vs. mock-HepG2
Project description:MicroRNAs are important cellular regulators and their dysfunctions are associated with various disease. miR-371/372/373 was found co-regulated in HBV-producing HepG2.2.15 cells when compared to its non-HBV producing maternal HepG2 cells. To obtain a glimpse of the potential influence of the enforced miR-371-372-373 cluster in HepG2 gene expression, a two-color Capitalbio 70-mer oligo microarray platform, which contained 21,329 well-characterized human gene probes, was used to identify the differentially expressed genes between miR-371-372-373-HepG2 and mock-HepG2 in two independent biological replicate.
Project description:Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths worldwide, with limited effective treatment options. Recent studies highlight the emerging role of microRNAs in HCC biology, including microRNA-372-3p (miR-372-3p), but its precise function in HCC remains controversial. Here, we investigated the role of miR-372-3p in HCC using a transcriptomic approach. Overexpression of miR-372-3p in HCC cell lines significantly impaired proliferation, migration, invasion, and colony formation, indicating a tumor-suppressive function. RNA sequencing and Gene Set Enrichment Analysis (GSEA) revealed downregulation of genes involved in fatty acid metabolism, specifically fatty acid oxidation (FAO). Consistently, miR-372-3p overexpression increased lipid droplet accumulation and triglyceride levels while inhibiting FAO, leading to impaired lipid utilization under glucose deprivation and compromised interactions of lipid droplets with mitochondria and lysosomes. Mechanistically, we identified CPT1A and ACSL4 as direct targets of miR-372-3p using bioinformatics and dual luciferase assays. These findings demonstrate that miR-372-3p acts as a tumor suppressor in HCC by inhibiting FAO and disrupting lipid metabolism, suggesting a potential therapeutic target for HCC treatment.
Project description:Proteomics of HEPG2 cells following FTO overexpression and knockdown. Data accompany our paper entitled “Dynamic Regulation of N6,2′-O-dimethyladenosine (m6Am) in Obesity” scheduled for publication in Nature Communications, 2021
Project description:To identify putative miRNA targets for LINC01126, we overexpressed LINC01126 in HepG2 cells and carried out a microarray analysis. A total of 66 miRNAs present in the array demonstrated 1.5-fold upregulation or downregulation of expression upon LINC01126 transfection.