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Naloxone influences NSCs proliferation and differentiation

ABSTRACT: Naloxone influences NSCs proliferation and differentiation

PROVIDER: PRJNA416497 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6238370_1.fastq.gz	Fastqsanger.gz
	SRR6238370_2.fastq.gz	Fastqsanger.gz
	SRR6238380_1.fastq.gz	Fastqsanger.gz
	SRR6238380_2.fastq.gz	Fastqsanger.gz
	SRR6238371_1.fastq.gz	Fastqsanger.gz

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Naloxone influences NSCs proliferation and differentiation

Project description:Naloxone is an opioid-receptor antagonist used to reverse the effects of opioid.In our study, we treated NSCs (neural stem cells) with naloxone and damgo (a selective μopioid receptor agonist), and examined their effects on NSCs proliferation and differentiation.Naloxone was found to promote NSCs proliferation and neural differentiation, while the effects of damgo was very week. The results suggested that naloxone may work indepedent of opioid receptors. To investigate the underlying mechanisms, RNA-seq was conducted. For proliferation analysis,p8 NSCs were cultured with or without naloxone or damgo for 3 days. For differentiation analysis,p8 NSCs was induced to differentiate with or without naloxone or damgo, samples on D2,4,8 were collected and analyzed.

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Project description:DNA methylation is known to regulate cell differentiation and neuronal function in vivo. Here we examined whether deficiency of a de novo DNA methyltransferase, Dnmt3a, affects in vitro differentiation of mouse embryonic stem cells (mESCs) to neuronal and glial cell lineages. We found that Dnmt3a-/- neural stem cells (NSCs) derived from mESCs have globally reduced methylcytosine levels and precociously differentiates into astrocytes and oligodendrocytes, consistent with our previous findings in the more severely hypomethylated Dnmt1-/- NSCs. Moreover, Dnmt3a-/- NSC proliferation rate was significantly increased when compared to control. Thus, our work revealed a novel role for Dnmt3a in regulating both timing of neural cell differentiation and cell proliferation in NSCs. Dnmt3a KO vs. WT neural stem cells; 3 biological replicates of each.

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Gene expression data from mouse ES cell-derived primitive NSCs and definitive NSCs

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