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Alicycliphilus denitrificans BC strain:BC

ABSTRACT: Alicycliphilus denitrificans BC whole genome sequencing

PROVIDER: PRJNA41663 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR058391.fastq.gz	Fastqsanger.gz
	SRR058392.fastq.gz	Fastqsanger.gz
	SRR058393.fastq.gz	Fastqsanger.gz
	SRR066080.fastq.gz	Fastqsanger.gz

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Similar Datasets

Proteomic response of Alicycliphilus denitrificans strain BC towards the different electron acceptors chlorate, nitrate and oxygen.

Project description:Alicycliphilus denitrificans is a versatile, ubiquitous, facultative anaerobic bacterium. A. denitrificans strain BC can use chlorate, nitrate and oxygen as electron acceptor for growth. Cells display a prolonged lag-phase when transferred from nitrate to chlorate and vice versa. Furthermore, cells adapted to aerobic growth do not easily use nitrate or chlorate as electron acceptor. We further investigated these responses of strain BC by differential proteomics, transcript analysis and enzyme activity assays. In nitrate-adapted cells transferred to chlorate and vice versa, appropriate electron acceptor reduction pathways need to be activated. In oxygen-adapted cells, adaptation to the use of chlorate or nitrate is likely difficult due to the poorly active nitrate reduction pathway and low active chlorate reduction pathway. We deduce that the Nar-type nitrate reductase of strain BC also reduces chlorate, which may result in toxic levels of chlorite if cells are transferred to chlorate. Furthermore, the activities of nitrate reductase and nitrite reductase appear to be not balanced when oxygen-adapted cells a shifted to nitrate as electron acceptor, leading to the production of a toxic amount of nitrite. These data suggest that strain BC encounters metabolic challenges in environments with fluctuations in the availability of electron acceptors. Proteomic samples were prepared from Alicycliphilus denitrificans grown in the presence of different electron acceptors: chlorate, nitrate and oxygen. Proteins were separated by a short SDS gel and for each sample 4 in-gel digest slices were prepared. Peptide samples were measured by nLC LTQ-Orbitrap and the data were analysed with MaxQuant using default settings (1% FDR on peptide and protein level) and filtered extra to keep only proteins identified with at least 2 peptides and 1 unique and 1 unmodified peptide.

2013-08-12 | PXD000258 | Pride

Alicycliphilus denitrificans

Project description:Alicycliphilus denitrificans overview

| PRJNA41697 | ENA

Alicycliphilus denitrificans strain:Ylb10

Project description:Alicycliphilus denitrificans strain:Ylb10 Genome sequencing

| PRJNA950334 | ENA

Alicycliphilus denitrificans strain:DP3

Project description:Alicycliphilus denitrificans strain:DP3 Genome sequencing

| PRJNA624042 | ENA

Alicycliphilus denitrificans K601

Project description:Alicycliphilus denitrificans K601 genome sequencing project.

| PRJNA50751 | ENA

Alicycliphilus denitrificans strain:CD02

Project description:Alicycliphilus denitrificans strain:CD02 Genome sequencing and assembly

| PRJNA417200 | ENA

Alicycliphilus denitrificans strain:BQ1

Project description:Alicycliphilus denitrificans strain:BQ1 Genome sequencing and assembly

| PRJNA391731 | ENA

Alicycliphilus denitrificans strain:I51

Project description:Genome analysis project of Alicycliphilus denitrificans strain I51

| PRJDB10822 | ENA

Alicycliphilus denitrificans strain:I51

Project description:RNA-Seq based Gene Expression Analysis of Alicycliphilus denitrificans strain I51

| PRJDB12083 | ENA

Genome sequences of Alicycliphilus denitrificans strains BC and K601T.

Project description:Alicycliphilus denitrificans strain BC and A. denitrificans strain K601(T) degrade cyclic hydrocarbons. These strains have been isolated from a mixture of wastewater treatment plant material and benzene-polluted soil and from a wastewater treatment plant, respectively, suggesting their role in bioremediation of soil and water. Although the strains are phylogenetically closely related, there are some clear physiological differences. The hydrocarbon cyclohexanol, for example, can be degraded by strain K601(T) but not by strain BC. Furthermore, both strains can use nitrate and oxygen as an electron acceptor, but only strain BC can use chlorate as electron acceptor. To better understand the nitrate and chlorate reduction mechanisms coupled to the oxidation of cyclic compounds, the genomes of A. denitrificans strains BC and K601(T) were sequenced. Here, we report the complete genome sequences of A. denitrificans strains BC and K601(T).

| S-EPMC3165661 | biostudies-literature

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