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Analysis of DNA methylation in colorectal cancer DLD1 cells with UHRF1 depletion and HDAC inhibition

ABSTRACT: Analysis of DNA methylation in colorectal cancer DLD1 cells with UHRF1 depletion and HDAC inhibition

PROVIDER: PRJNA416878 | ENA |

REPOSITORIES: ENA

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Analysis of DNA methylation in colorectal cancer DLD1 cells with UHRF1 depletion and HDAC inhibition

Project description:UHRF1 is a major regulator of epigenetic mechanism and is overexpressed in various human malignancies. In this study, we examined the involvement of UHRF1 in aberrant DNA methylation in colorectal cancer (CRC). In CRC cells, transient UHRF1 knockdown rapidly induced DNA demethylation across entire genomic regions, including CpG islands, gene bodies and repetitive elements. Nonetheless, UHRF1 depletion only minimally reversed CpG island hypermethylation-associated gene silencing. However, the combination of UHRF1 depletion and histone deacetylase (HDAC) inhibition synergistically reactivated the silenced genes and strongly suppressed CRC cell proliferation. Our results suggest that (i) maintenance of DNA methylation in CRC cells is highly dependent on UHRF1; (ii) UHRF1 depletion rapidly induces DNA demethylation, though it is insufficient to fully reactivate the silenced genes; and (iii) dual targeting of UHRF1 and HDAC may be an effective new therapeutic strategy.

2019-05-21 | GSE106437 | GEO

Analysis of gene expression in colorectal cancer DLD1 cells with UHRF1 depletion and HDAC inhibition

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Analysis of gene expression in colorectal cancer DLD1 cells with UHRF1 depletion and HDAC inhibition

Project description:Analysis of gene expression in colorectal cancer DLD1 cells with UHRF1 depletion and HDAC inhibition

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Analysis of DNA methylation in colorectal cancer RKO cells with UHRF1 depletion and HDAC inhibition

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Analysis of gene expression in colorectal cancer RKO cells with UHRF1 depletion and HDAC inhibition

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UHRF1 depletion and HDAC inhibition synergistically reactivate epigenetically silenced genes in colorectal cancer cells

Project description:UHRF1 depletion and HDAC inhibition synergistically reactivate epigenetically silenced genes in colorectal cancer cells

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Analysis of gene expression in colorectal cancer RKO cells with UHRF1 depletion and HDAC inhibition

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Analysis of DNA methylation in colorectal cancer RKO cells with UHRF1 depletion and HDAC inhibition

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Gene expression changes upon Lamin A and Lamin B2 depletion in a diploid colorectal cancer cell line - DLD1

Project description:Small interfering RNA (siRNA) mediated depletion of Lamin A/C and Lamin B2 was performed in a diploid colorectal cancer cell line - DLD1 with an aim to identify transcription deregulation across the genome.

2016-09-24 | GSE73269 | GEO

Effect of depletion of ZNF692 on gene expression in DLD1 colorectal human cells

Project description:To investigate the function of ZNF692 in colorectal cancer, we established DLD1 cell lines in which target gene has been knocked out by CRISPR-cas. We then performed gene expression profiling analysis using data obtained from DLD1 colorectal human cells wt (sgC for guide control) or crispr knockout for ZNF692 (sgZ1 for guide 1, and sgZ3 for guide 3). We have each condition in triplicates.

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