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Drosophila melanogaster strain:W1118

ABSTRACT: Expression profiling by high throughput sequencing among wild type and mt2 null before and after infection

PROVIDER: PRJNA417173 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6277505_1.fastq.gz	Fastqsanger.gz
	SRR6277505_2.fastq.gz	Fastqsanger.gz

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mRNA sequence of individual Drosophila melanogaster (w1118) male and female flies fed with different concentrations of dimethyl sulfoxide (DMSO) over different time periods.

Project description:To gain an understanding of the toxic effect of a commonly used solvent, flies were exposed to 0, 0.1, 0.2, or 0.4% v/v DMSO for 0, 2, 4, or 8 h. We performed compound exposure of 800 individual flies in 4 Whole Animal Feeding Flats (WAFFL), a novel 96 well system to house, feed, and harvest individual flies. This expression profiling was part of a set of the experiments performed to evaluate the suitability of the WAFFL for high throughput small compound screening in D. melanogaster. Treated flies and controls were used for poly A+ stranded mRNA library preparation and we performed high throughput RNA sequencing to determine the transcriptional changes due to DMSO treatment.

2017-05-05 | GSE81142 | GEO

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