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Expression data from TabZIP6-overexpressed Arabidopsis before/after cold treatment

ABSTRACT: Expression data from TabZIP6-overexpressed Arabidopsis before/after cold treatment

PROVIDER: PRJNA417485 | ENA |

REPOSITORIES: ENA

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Expression data from TabZIP6-overexpressed Arabidopsis before/after cold treatment

Project description:TabZIP6 is a Subgroup C bZIP protein from wheat. Some evidences have shown that TabZIP6 is involved in cold stress response. To elucidate the regulatory mechanism of TabZIP6 in plant cold response, cold treated/untreated TabZIP6-overexpressing Arabidopsis seedlings were used to perform microarray analysis.

2018-01-01 | GSE106635 | GEO

Expression data in Arabidopsis Col-0 with cold acclimation (CA) treatment and subsequent cold deacclimation (DA) treatment.

Project description:To understand mRNA expression pattern during cold acclimation and deacclimation, transcriptional profiling of cold acclimation and deacclimation-treated plants were analyzed using Agilent-015059 Arabidopsis 3 Oligo Microarray 4x44K G2519F. Arabidopsis Col-0 were grown on MS plate for 2 weeks (16 hours light / 8 hours dark). Two week-old Arabidopsis samples (NA, non acclimation) were treated with cold (2 M-BM-:C) for 7 days (CA7d) under 12h/12h light/dark conditions. Deacclimation-treated samples (DA6h, DA12h, DA24h) were grown at normal growth temperature under long day conditions after cold treatment for 7 days. Then total RNA was prepared from the whole seedling and used for the microarray hybridization. Three replicative hybridization experiments for each array were carried out using the independent biological samples.

2014-10-02 | E-GEOD-49796 | biostudies-arrayexpress

Expression data in Arabidopsis Col-0 with cold acclimation (CA) treatment and subsequent cold deacclimation (DA) treatment.

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Transcriptome differences between wild-type and ERF096 transgenic rice before and after cold treatment

Project description:To reveal the involvement of OsERF096 in response to cold stress, this study performed transcriptome sequencing of WT, OsERF096-OX and OsERF096-RNAi rice before and after cold stress.Based on transcriptome sequencing, this study found that OsERF096 is involved in the regulation of hormone signal transduction.

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Transcriptome differences between wild-type and ERF096 transgenic rice before and after cold treatment

Project description:Transcriptome differences between wild-type and ERF096 transgenic rice before and after cold treatment

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Transcription profiling of Arabidopsis wild type and ice1 mutant plants exposed to cold treatment

Project description:To understand the gene network that controls plant tolerance to cold stress, we carried out a near full genome transcript expression profiling in Arabidopsis using Affymetrix GeneChips that contain approximately 24,000 genes. For microarray analysis, Arabidopsis seedlings were cold treated at 0 C for 0 h, 3 h, 6 h, and 24 h. A total of 939 genes were statistically determined to be cold-regulated with 655 being up-regulated and 284 down-regulated. A large number of the early cold-responsive genes encode transcription factors that likely control late-responsive genes, which implies a multitude of transcriptional cascades. In addition, many genes involved in post-transcriptional and chromatin level regulation were also cold regulated suggesting their involvement in cold responsive gene regulation. A number of genes important for the biosynthesis or signaling of plant hormones, such as abscisic acid, gibberellic acid and auxin, are regulated by cold stress, which is of potential importance in coordinating cold tolerance with growth and development. We compared the cold-responsive transcriptomes of wild type and ice1, a mutant defective in an upstream transcription factor required for chilling and freezing tolerance. The transcript levels of many cold-responsive genes were altered in the ice1 mutant not only during cold stress conditions, but also before cold treatments. Our study provides a global picture of the Arabidopsis cold-responsive transcriptome and its control by ICE1, and thus will be valuable for understanding gene regulation under cold stress and the molecular mechanisms of cold tolerance. Experiment Overall Design: Two replicates for each time point of 0 hours, 3 hours, 6 hours and 24 hours of cold treatment for the wildtype (control) and ice1 (mutant).

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RNA seq before and after cold pressor test

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Expression data for drought-, cold, high-salinity and ABA treatment in Arabidopsis plants

Project description:We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome under drought, cold, high-salinity and ABA treatment conditions and idenfied many stress- or ABA- responsive putative functional RNAs and fully-overlapping sense-antisense transcripts in Arabidopsis genome. Keywords: stress response Two-week-old Arabidopsis plants grown on the agar plates were subjected to the stress- or ABA- treatments. The total RNA was prepared from the treated- and untreated- plants, and used for the microarray hybridization. Three replicative hybridization experiments for each strand array were carried out using the independent biological RNA samples.

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Transcription profiling of nine ecotypes of Arabidopsis before and after 14d of cold acclimation at 4 degrees C

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