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Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [EBF1-ChIP-Seq-Cre]

ABSTRACT: Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [EBF1-ChIP-Seq-Cre]

PROVIDER: PRJNA419400 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6313860_1.fastq.gz	Fastqsanger.gz
	SRR6313860_2.fastq.gz	Fastqsanger.gz
	SRR6313861_1.fastq.gz	Fastqsanger.gz
	SRR6313861_2.fastq.gz	Fastqsanger.gz
	SRR6313862_1.fastq.gz	Fastqsanger.gz

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Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [EBF1-ChIP-Seq-Cre]

Project description:EBF1 is essential for B cell specification and commitment. To explore the dynamics of EBF1 initiated B cell programming, we performed EBF1 ChIP-seq, ATAC-seq, bisulfite-seq, RNA-seq and several histone ChIP-seq analyses at different stages of the transition from Ebf1-/- pre-pro-B to pro-B triggered by EBF1 restoration. We also performed Pax5 ChIP-seq in Ebf1-/- pre-pro-B cell and EBF1-restored pro-B cell to study the pioneering function of EBF1 that allows other transcription factors to access certain chromatin sites.

2018-02-20 | GSE107236 | GEO